RIOK3 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1.
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Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1
Alternative names
Homolog of the Aspergillus nidulans sudD gene product, RIO kinase 3, RIO kinase 3 (yeast), RIOK3_HUMAN, SUDD, Serine/threonine-protein kinase RIO3, SudD (suppressor of bimD6, Aspergillus nidulans) homolog, SudD suppressor of Aspergillus nidulans bimD6 homolog, SudD suppressor of bimD6 homolog, sudD homolog
Recommended products
RIOK3 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- RIOK3
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RIOK3 also known as RIO kinase 3 or SUDD is a serine/threonine kinase with a molecular mass of approximately 65 kDa. This protein acts as an atypical kinase within the RIO kinase family and localizes to the cytoplasm. RIOK3 shows expression in various tissues with higher levels notably in the immune system including the spleen and thymus. Unlike typical kinases RIOK3 contains a unique winged-helix domain that contributes to its diverse functions in cellular processes.
- Biological function summary
RIOK3 participates in immune response regulation and cellular signal transduction. It does not form part of any known large protein complex but interacts with several other proteins through transient associations. By modulating pathways involved in immune processes RIOK3 affects the activation state of various immune cells. It is involved in the regulation of interferon production where it works closely with other signaling proteins to modulate responses to viral infections.
- Pathways
RIOK3 serves a role in the innate immune response and RNA processing pathways. It influences the retinoic acid-inducible gene I (RIG-I) pathway where it interacts with proteins such as RIG-I and MAVS to mediate antiviral responses. Additionally RIOK3 plays a modulatory role in mRNA processing connecting with proteins involved in RNA maturation and internationalization which suggests its involvement in maintaining proper RNA homeostasis under stress conditions.
- Associated diseases and disorders
RIOK3 has associations with autoimmune conditions and viral infections highlighting its role in immune regulation. Aberrant functioning of RIOK3 can relate to systemic lupus erythematosus (SLE) where it influences the overproduction of interferons exacerbating the disease phenotype. Furthermore RIOK3 modulates responses to hepatitis C virus infections interacting with proteins such as RIG-I to control viral replication and immune evasion. Understanding RIOK3's functions can lead to insights into therapeutic interventions for these conditions.
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1 product image
Sanger Sequencing - Human RIOK3 knockout HeLa cell line (ab264774)
Homozygous: Insertion of the selection cassette in exon 1.
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