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AB280065

Human RIPK3 knockout THP-1 cell line

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RIPK3 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.
1 Images
Western blot - Human RIPK3 knockout THP-1 cell line (AB280065)
  • WB

Lab

Western blot - Human RIPK3 knockout THP-1 cell line (AB280065)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Performed under reducing conditions.

In Western blot, ab325636 was shown to bind specifically to RIPK3. Target of interest was observed at 37-50 kDa in wild-type THP-1 cell lysates (lane 1) with no signal observed at this size in RIPK3 knockout cell line (lane 2)(knockout cell line ab280065).

In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) (1 : 100000) (17KDa).

All lanes:

Western blot - Anti-RIP3 antibody [RM1355] (ab325636) at 1/1000 dilution

Lane 1:

Wilde-type THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

Lane 2:

Western blot - Human RIPK3 knockout THP-1 cell line (ab280065) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 37-56 kDa,17 kDa

false

Exposure time: 180s

Key facts

Cell type

THP-1

Species or organism

Human

Tissue

Blood

Form

Liquid

form

Knockout validation

Sanger Sequencing,Western blot

Disease

Carcinoma

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Recommended control: Human wild-type THP-1 cell line (ab281894). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.

  • Upon thawing make banks as soon as possible and use each bank within 10-20 passages
  • As cell line growth can vary please attempt culture for 2 weeks from revival of initial bank before contacting the technical team.
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What's included?

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Properties and storage information

Gene name
RIPK3
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-196°C
Appropriate long-term storage conditions
-196°C
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Suspension

Gender

Male

Viability

~ 60%

Subculture guidelines
  • All seeding densities should be based on cell counts gained by established methods.
  • Cells should be seeded at 2x105 - 3x105 cells/mL and subcultured when they have reached 8x105 cells/mL.
  • It is not recommended to allow the cell density to exceed 1x106 cells/mL.
Culture medium

RPMI + 10% FBS + 0.05 mM beta-mercaptoethanol

Cryopreservation medium

Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

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Product protocols

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Product promise

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For full details, please see our Terms & Conditions

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