RMDN3 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon 2 and 1 bp insertion in exon 2.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon 2 and 1 bp insertion in exon 2
Alternative names
Cerebral protein 10, FAM82C, FAM82C, formerly, FLJ10579, Family with sequence similarity 82 member C, Family with sequence similarity 82 member C, formerly, Family with sequence similarity 82, member A2, Microtubule Associated Protein, Protein FAM82A2, Protein FAM82C, Protein tyrosine phosphatase interacting protein 51, Regulator of microtubule dynamics 3, Regulator of microtubule dynamics protein 3, TCPTP interacting protein 51, hRMD 3
Recommended products
RMDN3 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon 2 and 1 bp insertion in exon 2.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon 2 and 1 bp insertion in exon 2
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- RMDN3
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PTPIP51 also known as Protein Tyrosine Phosphatase-Interacting Protein 51 functions at a mechanical level by interacting with protein tyrosine phosphatases and kinases which are key regulators of signal transduction. PTPIP51 has a molecular mass of approximately 51 kDa. The protein is expressed in various tissues including the brain testis and skeletal muscle. Its interactions suggest a role in maintaining cellular function and signaling.
- Biological function summary
The PTPIP51 protein plays a role in cellular processes such as apoptosis and cellular differentiation. It is believed to associate with other proteins to form complexes which influence mitochondrial function and cellular energy metabolism. Through these interactions PTPIP51 aids in maintaining cellular homeostasis and regulating cell survival mechanisms.
- Pathways
PTPIP51 participates in the regulation of the MAPK/ERK pathway and the insulin signaling pathway. It interacts with proteins such as RAF1 and GRB2 to convey signals from the cell surface to the DNA in the cell nucleus affecting cell division and proliferation. These pathways highlight the protein’s importance in controlling cellular growth and reaction to external stimuli.
- Associated diseases and disorders
PTPIP51 has connections to neurodegenerative disorders like Alzheimer's disease and metabolic conditions such as obesity. In Alzheimer’s alterations in PTPIP51 are linked with the dysregulation of mitochondrial function and it interacts with proteins such as Tau involved in neurofibrillary tangles. In obesity PTPIP51 is connected to insulin resistance engaging with protein partners like IRS1 to influence glucose uptake and metabolism.
Product promise
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2 product images
Sanger Sequencing - Human RMDN3 (PTPIP51) knockout HeLa cell line (ab265688)
Allele-2: 1 bp insertion in exon 2.
Sanger Sequencing - Human RMDN3 (PTPIP51) knockout HeLa cell line (ab265688)
Allele-1: 14 bp deletion in exon 2.
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