Human RPH3A knockout SK-N-FI cell line
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- Sanger seq
Supplier Data
Sanger Sequencing - Human RPH3A knockout SK-N-FI cell line (AB288709)
Homozygote. 43 bp deletion
Complementary Products
Reactivity data
Product details
Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
All seeding densities should be based on cell counts gained by established methods.
Culture medium
DMEM + 0.1 mM NEAA + 10 % FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Rabphilin 3A modulates synaptic vesicle docking and fusion in the neuron. It acts as an effector of the small GTPase Rab3 forming a complex that links synaptic vesicles to presynaptic membranes. This interaction influences the dynamics of the membrane and ensures proper vesicle cycling during neurotransmission. Rabphilin 3A also interacts with calcium ions which regulate its affinity for other proteins involved in the synaptic machinery.
Pathways
Rabphilin 3A participates in the regulation of the neurotransmitter release pathway and synaptic vesicle cycle. It interacts with proteins like Rab3A to facilitate vesicle docking and membrane fusion important for synaptic vesicle exocytosis. The cAMP-dependent signaling pathway also involves Rabphilin 3A guiding the processes associated with synapse communication and plasticity.
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com