RPRM KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 106 bp deletion in exon 1 and 53 bp deletion in exon 1.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 106 bp deletion in exon 1 and 53 bp deletion in exon 1
Alternative names
RPRM
Recommended products
RPRM KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 106 bp deletion in exon 1 and 53 bp deletion in exon 1.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 106 bp deletion in exon 1 and 53 bp deletion in exon 1
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- RPRM
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Protein reprimo (RPRM) also known as Reprimo is a small protein with a molecular mass of approximately 12.5 kDa. It does its mechanical function by regulating cell cycle arrest specifically at the G2/M checkpoint. RPRM localizes to the cytoplasm and is expressed in a varied range of tissues including the stomach and colon indicating its importance in gastrointestinal biology. It interacts with the cytoskeletal elements to exert its influence on cellular activities.
- Biological function summary
RPRM acts as a tumor suppressor by inhibiting cell proliferation mainly through its G2 arrest function. It is not formally known to be part of a larger protein complex but it works closely with p53 a well-known regulatory protein and tumor suppressor to mediate its effects. RPRM expression helps prevent abnormal cell growth under stress conditions contributing to the maintenance of genomic stability.
- Pathways
RPRM plays critical roles in the p53 signaling pathway and the cell cycle control pathway. In the p53 pathway RPRM aids in enforcing a cell cycle halt when DNA damage is detected working alongside other proteins such as p21 and GADD45. In the context of cell cycle control RPRM functions as an intermediary that facilitates the arrest of cell division in response to DNA stress signals collaborating with these proteins to enhance DNA repair mechanisms.
- Associated diseases and disorders
RPRM interactions significantly impact cancer particularly gastric cancer and colorectal cancer. The protein's reduced expression is frequently seen in malignancies where p53 pathway disruptions are apparent. In these cancers connections exist between the downregulation of RPRM and overexpression of Bcl-2 an anti-apoptotic protein where RPRM loss results in impaired apoptosis and uncontrolled cell survival. This association marks RPRM as a potential biomarker for understanding tumor dynamics in these types of cancers.
Product promise
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Full details and terms and conditions can be found here:
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2 product images
Sanger Sequencing - Human RPRM (Protein reprimo) knockout HeLa cell line (ab265810)
Allele-1: 53 bp deletion in exon 1.
Sanger Sequencing - Human RPRM (Protein reprimo) knockout HeLa cell line (ab265810)
Allele-2: 106 bp deletion in exon 1.
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