RRAGB KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 85 bp insertion in exon 1.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 85 bp insertion in exon 1
Alternative names
GTP-binding protein ragB, RRAGB_HUMAN, Rag B, Ras-related GTP-binding protein B
Recommended products
RRAGB KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 85 bp insertion in exon 1.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 85 bp insertion in exon 1
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- RRAGB
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RRAGB also known as Ras-related GTPase B is a member of the Rag family of GTPases. This protein has a molecular mass of approximately 42 kDa. RRAGB is expressed in various tissues with significant expression in the brain and skeletal muscles. The protein functions as a molecular switch toggling between active GTP-bound and inactive GDP-bound states. This cycling allows RRAGB to regulate mTORC1 signaling which is essential for cellular growth and metabolism.
- Biological function summary
RRAGB partners with other Rag GTPases to form heterodimeric complexes. These complexes facilitate the proper localization and activation of mTORC1 on the lysosomal membrane. Moreover RRAGB plays a role in amino acid sensing which impacts cellular growth. By responding to intracellular nutrient levels RRAGB helps modulate anabolic processes and energy balance.
- Pathways
RRAGB is integral to the mTOR signaling and nutrient-sensing pathways. As a part of these pathways RRAGB collaborates with proteins such as Raptor and Rheb. These interactions ensure efficient transmission of signals that regulate cell growth and metabolism. The accurate function of this network supports cellular adaptation to varying environmental nutrient conditions.
- Associated diseases and disorders
RRAGB has been associated with certain cancers and neurodegenerative diseases. Alterations in the mTOR pathway involving RRAGB can contribute to undesired cellular proliferation observed in cancers. Moreover disruptions in RRAGB activity can influence mTORC1 signaling in neurodegenerative conditions. It's important to further understand how RRAGB and related proteins like mTOR are involved in the pathology of these diseases to develop targeted therapies.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Sanger Sequencing - Human RRAGB knockout HeLa cell line (ab264761)
Allele-2: 85 bp insertion in exon 1.
Sanger Sequencing - Human RRAGB knockout HeLa cell line (ab264761)
Allele-1: 1 bp insertion in exon1
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