RRP36 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 19 bp deletion in exon 1 and 1 bp insertion in exon 1.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 19 bp deletion in exon 1 and 1 bp insertion in exon 1
Alternative names
RRP36_HUMAN, Ribosomal RNA processing protein 36 homolog
Recommended products
RRP36 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 19 bp deletion in exon 1 and 1 bp insertion in exon 1.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 19 bp deletion in exon 1 and 1 bp insertion in exon 1
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- RRP36
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RRP36 also known as RRP36 homolog is a protein involved in ribosome biogenesis. It has a molecular mass of approximately 33 kDa. This protein is expressed chiefly in the nucleolus of eukaryotic cells. It plays an important mechanical role in the early stages of processing of precursor rRNA. RRP36 is essential for the maturation of the 90S pre-ribosome an important step in ribosome synthesis.
- Biological function summary
The RRP36 protein functions as part of the small subunit (SSU) processome a large ribonucleoprotein complex. This complex plays a significant role in the early cleavage steps of pre-rRNA necessary for forming functional ribosomes. By contributing to pre-rRNA processing RRP36 aids in the efficient production of ribosomal RNA a core component of ribosomes. This precise processing is required for ribosome assembly and cellular protein synthesis.
- Pathways
RRP36 integrates into the nucleolar small subunit processome-related pathway influential in ribosome biogenesis. This pathway involves many proteins including UTP4 and UTP15 which closely associate with RRP36 in the SSU processome. The successful operation of this pathway is critical for linking ribosome production to overall cellular growth proliferation and metabolism.
- Associated diseases and disorders
RRP36 shows a connection with disorders resulting from defects in ribosome biogenesis. Impaired ribosome production can contribute to ribosomopathies which include diseases such as Diamond-Blackfan anemia. In this context RRP36 operates alongside other ribosomal biogenesis-related proteins such as UTP18 emphasizing its role in normal cellular functioning and the consequences arising from its dysregulation.
Product promise
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2 product images
Sanger Sequencing - Human RRP36 knockout HeLa cell line (ab266019)
Allele-1: 19 bp deletion in exon 1.
Sanger Sequencing - Human RRP36 knockout HeLa cell line (ab266019)
Allele-2: 1 bp insertion in exon 1.
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