Human RTN4RL2 (NgR2) knockout HeLa cell line
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Human RTN4RL2 (NgR2) knockout HeLa cell line available to order. Recommended control: Human wild-type HeLa cell line (ab255928).
View Alternative Names
NGRH1, NGRL3, NgR2, Nogo receptor-like 3, Nogo-66 receptor homolog 1, Nogo-66 receptor-related protein 2, R4RL2_HUMAN, RTN4RL2, Reticulon-4 receptor-like 2
- Sanger seq
Unknown
Sanger Sequencing - Human RTN4RL2 (NgR2) knockout HeLa cell line (AB265229)
Homozygous : 10 bp deletion in exon 2.
Product details
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The action of NgR2 impacts the neural regeneration processes by forming a receptor complex with p75 neurotrophin receptor (p75NTR) and LINGO-1. This complex activation inhibits axonal outgrowth and neural plasticity which affects the nervous system's ability to recover after injury. NgR2 mediates signaling that prevents neurite outgrowth contributing to the stabilization of neuronal connections in postnatal brain development. NgR2's role in these processes makes it a significant target for studies investigating neuronal repair mechanisms.
Pathways
Axon guidance and myelin inhibition play significant roles in the function of NgR2. It participates in the RhoA-ROCK signaling pathway which is activated upon myelin ligand binding to the receptor complex. This pathway is responsible for cytoskeletal changes that inhibit neurite extension. Additionally NgR2 interacts with proteins such as Nogo-A and MAG further influencing axonal growth inhibition and regeneration in the nervous system.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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