RTP4 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 5 bp deletion in exon 2.
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Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 5 bp deletion in exon 2
Alternative names
28 kDa interferon-responsive protein, 28kD interferon responsive protein, IFRG28, RTP4_HUMAN, Receptor (chemosensory) transporter protein 4, Receptor transporter protein 4, Receptor-transporting protein 4, Z3CXXC4, Zinc finger, 3CxxC type 4
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RTP4 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 5 bp deletion in exon 2.
Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 5 bp deletion in exon 2
- Disease
- Carcinoma
- Concentration
Properties
- Gene name
- RTP4
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 1 US: 1
- Adherent/suspension
- Adherent
- Gender
- Male
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Do not allow the cell density to exceed 7x104 cells/cm2.
- Culture medium
- F-12K + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RTP4 also known as Receptor Transporter Protein 4 plays a significant role in the endoplasmic reticulum where it facilitates the trafficking and proper function of olfactory receptors. It has a molecular mass of approximately 31 kDa. RTP4 expression is mainly found in the olfactory sensory neurons but also in various other tissues such as the brain and liver. It helps in guiding G protein-coupled receptors (GPCRs) to the cell membrane ensuring their functional expression.
- Biological function summary
The RTP4 protein aids in the transport and functional expression of GPCRs without forming part of any known stable complexes. This chaperone activity is vital for GPCR function impacting signal transduction by promoting proper receptor folding and surface expression. RTP4 ensures that olfactory and other related receptors can adequately respond to stimuli. Its chaperone activity is important for maintaining GPCR dynamics in various cell types.
- Pathways
RTP4 significantly influences GPCR signaling pathways and olfactory signaling pathways. It directly affects the transport and activation of olfactory receptors key components in the olfactory signaling pathway. RTP4's interaction with olfactory receptors impacts the downstream components such as adenylyl cyclase leading to successful signal transduction. Additionally RTP4 interacts with proteins in GPCR signaling that modify receptor availability on the cell surface.
- Associated diseases and disorders
RTP4 has implications in anosmia the loss or impairment of the sense of smell and potentially in liver-related disorders. Disruption of RTP4 function can lead to defective olfactory receptor trafficking resulting in anosmia. RTP4-related pathways implicate proteins like OR51E1 in olfactory dysfunctions. There may be a less clear association with liver disorders involving misfolded GPCRs; however this link requires further exploration.
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1 product image
Sanger Sequencing - Human RTP4 knockout A549 cell line (ab267115)
Homozygous: 5 bp deletion in exon2
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