SATB1 KO cell line available to order. Free of charge wild type control provided.
A549
Human
Lung
Liquid
DNA-binding protein SATB1, SATB homeobox 1, SATB1_HUMAN, Special AT rich sequence binding protein 1 (binds to nuclear matrix/scaffold associating DNA), Special AT-rich sequence-binding protein 1
SATB1 KO cell line available to order. Free of charge wild type control provided.
A549
Human
Lung
Liquid
Carcinoma
SATB1
Knockout
CRISPR technology
EU: 1 US: 1
Adherent
Male
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Dry Ice
-196°C
-196°C
Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.
Recommended control: Human wild-type A549 cell line (Human wild-type A549 cell line ab288558). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
This supplementary information is collated from multiple sources and compiled automatically.
SATB1 or Special AT-rich Sequence Binding Protein 1 is a nuclear matrix-associated protein with a mass of approximately 85 kDa. It modifies chromatin structure by organizing the density of DNA through loop formation which regulates gene expression. SATB1 is expressed in thymocytes breast tissue and various other cell types and plays a significant role in cellular differentiation and development. It binds selectively to base-unpairing regions allowing it to influence expression patterns of multiple genes.
SATB1 acts as a genome organizer and transcription regulator by linking chromatin remodeling complexes to specific genomic loci. It participates in chromatin loop domain organization influencing higher-order chromatin structure and gene silencing or activation. SATB1 does not usually work alone but rather associates with corepressors such as HDAC1 and coactivators like PCAF in regulating transcription. Its functions have been noted as particularly important in T cell development where it assists in the regulation of T cell receptor genes.
SATB1 integrates into the Wnt signaling pathway and influences the Notch signaling pathway. The protein interacts with β-catenin in the Wnt pathway aiding in sustaining key developmental processes. In the Notch signaling pathway SATB1 influences cell fate decisions impacting differentiation and proliferation through its interaction with RBP-Jκ and other transcription modulators. MUTYH and LRP6 are among the proteins within these pathways influenced by SATB1's regulatory capacity.
SATB1 links to various cancer types including breast cancer and colorectal cancer. Overexpression of SATB1 can drive tumor progression and metastasis by altering gene expression landscapes. It's known to affect pathways regulated by proteins like BRCA1 in breast cancer contributing to disease pathogenicity. In immune disorders such as autoimmune diseases SATB1 interacts with STAT5 impacting cytokine signaling pathways and thereby influencing immunological tolerance and efficacy.
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