SCAF11 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 10 and 2 bp deletion in exon 10.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 10 and 2 bp deletion in exon 10
Alternative names
1110061H03Rik, 2610510E10Rik, AI462454, CASP-11, CTD associated SR protein 11, Renal carcinoma antigen NY REN 40, SC35 interacting protein 1, SFRS2IP, SIP-1, SRrp129, Splicing regulatory protein 129, mKIAA3013, splicing factor, arginine/serine rich 2, interacting protein
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SCAF11 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 10 and 2 bp deletion in exon 10.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 10 and 2 bp deletion in exon 10
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- SCAF11
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SFRS2IP also known as SFRS2-interacting protein and Cisplatin Resistance-Associated Overexpressed Protein (CRISP-1) is a protein with a relative mass of around 92 kilodaltons. It primarily interacts with the serine/arginine-rich splicing factor 2 (SFRS2) as indicated by its name. Researchers have found SFRS2IP mainly in the nucleus of cells where it plays a significant role in pre-mRNA splicing. Studies show its expression in various tissues but it is especially present in actively dividing cells.
- Biological function summary
Proteins like SFRS2IP contribute to the regulation of RNA processing and stability. SFRS2IP participates in the spliceosome complex facilitating correct splicing and maturation of precursor mRNA into mature RNA. This process is essential for gene expression regulation ensuring that proteins are synthesized properly and in necessary amounts. SFRS2IP's involvement in these processes influences cell cycle progression and can impact cellular responses to environmental changes.
- Pathways
Several studies have included SFRS2IP in processes related to mRNA splicing and gene expression. It is closely connected to the spliceosome pathway where it cooperates with SFRS2 and other splicing factors contributing to proper intron removal and exon joining in precursor mRNA. The protein also interacts with components of the RNA transport pathway ensuring the correct delivery of mRNA to the ribosome for translation.
- Associated diseases and disorders
Scientists have linked SFRS2IP to cancer and drug resistance mechanisms. Elevated expression levels of this protein have been observed in certain neoplastic tissues suggesting a role in tumorigenesis. It is associated in pathways involving oncogenic proteins like SFRS2 which influence cell proliferation and survival. Additionally studies have highlighted a connection between this protein and cisplatin resistance where SFRS2IP may interact with DNA repair proteins to modulate the cellular response to chemotherapy.
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2 product images
Sanger Sequencing - Human SCAF11 (SFRS2IP) knockout HeLa cell line (ab265343)
Allele-1: 2 bp deletion in exon 10.
Sanger Sequencing - Human SCAF11 (SFRS2IP) knockout HeLa cell line (ab265343)
Allele-2: 1 bp deletion in exon 10.
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