Human SCAF11 (SFRS2IP) knockout HeLa cell line
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SCAF11 KO cell line available to order. KO validated. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 10 and 2 bp deletion in exon 10. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.
View Alternative Names
1110061H03Rik, 2610510E10Rik, AI462454, CASP-11, CTD associated SR protein 11, Renal carcinoma antigen NY REN 40, SC35 interacting protein 1, SFRS2IP, SIP-1, SRrp129, Splicing regulatory protein 129, mKIAA3013, splicing factor, arginine/serine rich 2, interacting protein
- Sanger seq
Unknown
Sanger Sequencing - Human SCAF11 (SFRS2IP) knockout HeLa cell line (AB265343)
Allele-1 : 2 bp deletion in exon 10.
- Sanger seq
Unknown
Sanger Sequencing - Human SCAF11 (SFRS2IP) knockout HeLa cell line (AB265343)
Allele-2 : 1 bp deletion in exon 10.
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Proteins like SFRS2IP contribute to the regulation of RNA processing and stability. SFRS2IP participates in the spliceosome complex facilitating correct splicing and maturation of precursor mRNA into mature RNA. This process is essential for gene expression regulation ensuring that proteins are synthesized properly and in necessary amounts. SFRS2IP's involvement in these processes influences cell cycle progression and can impact cellular responses to environmental changes.
Pathways
Several studies have included SFRS2IP in processes related to mRNA splicing and gene expression. It is closely connected to the spliceosome pathway where it cooperates with SFRS2 and other splicing factors contributing to proper intron removal and exon joining in precursor mRNA. The protein also interacts with components of the RNA transport pathway ensuring the correct delivery of mRNA to the ribosome for translation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB68481
20ul selling size|RabMAb|Recombinant|KO Validated
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- 5
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com