Human SCD (SCD1) knockout HeLa cell line
- Advanced Validation
- What is this?
Be the first to review this product! Submit a review
|
(1 Publication)
SCD KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 4 bp deletion in exon 3 and Insertion of the selection cassette in exon 3. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.
View Alternative Names
ACOD_HUMAN, Acyl-CoA desaturase, Delta(9)-desaturase, Delta-9-Desaturase, FADS5, Fatty acid desaturase, PRO0998, Stearoyl-CoA desaturase
- WB
Lab
Western blot - Human SCD (SCD1) knockout HeLa cell line (AB265220)
anes 1-3 : Merged signal (red and green). Green - ab236868. Red - loading control ab8245 observed at 50 kDa.
ab236868 Anti-SCD1 antibody [EPR21963] was shown to specifically react with SCD1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265220 (knockout cell lysate ab257658) was used. Wild-type and SCD1 knockout samples were subjected to SDS-PAGE. ab236868 and Anti-tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4° at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-SCD1 antibody [EPR21963] (<a href='/en-us/products/primary-antibodies/scd1-antibody-epr21963-ab236868'>ab236868</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
SCD knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human SCD (SCD1) knockout HeLa cell line (ab265220)
Lane 3:
HepG2 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 42 kDa
false
- WB
Lab
Western blot - Human SCD (SCD1) knockout HeLa cell line (AB265220)
Lanes 1 - 4 : Merged signal (red and green). Green - ab19862 observed at 36 kDa. Red - loading control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55kDa.
ab19862 was shown to react with SCD1 in wild-type HeLa cells in western blot with loss of signal observed in SCD knockout cell line ab265220 (SCD knockout cell lysate ab257658). Wild-type and SCD knockout HeLa cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab19862 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4° at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-SCD1 antibody [CD.E10] (<a href='/en-us/products/primary-antibodies/scd1-antibody-cde10-ab19862'>ab19862</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
SCD knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human SCD (SCD1) knockout HeLa cell line (ab265220)
Lane 3:
HepG2 cell lysate at 20 µg
Predicted band size: 42 kDa
Observed band size: 36 kDa
false
- WB
Lab
Western blot - Human SCD (SCD1) knockout HeLa cell line (AB265220)
ab236868 was shown to react with SCD1 in wild-type HeLa cells in Western blot with loss of signal observed in SCD1 knockout cell line ab265220. Wild-type HeLa and SCD1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab236868 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.
These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
All lanes:
Western blot - Anti-SCD1 antibody [EPR21963] (<a href='/en-us/products/primary-antibodies/scd1-antibody-epr21963-ab236868'>ab236868</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa lysate at 35 µg
Lane 2:
SCD1 knock-out HeLa lysate at 35 µg
false
- WB
Lab
Western blot - Human SCD (SCD1) knockout HeLa cell line (AB265220)
ab19862 was shown to react with SCD1 in wild-type HeLa cells in Western blot with loss of signal observed in SCD1 knockout cell line ab265220. Wild-type HeLa and SCD1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab19862 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.
These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
All lanes:
Western blot - Anti-SCD1 antibody [CD.E10] (<a href='/en-us/products/primary-antibodies/scd1-antibody-cde10-ab19862'>ab19862</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa lysate at 35 µg
Lane 2:
SCD1 knock-out HeLa lysate at 35 µg
false
- Sanger seq
Unknown
Sanger Sequencing - Human SCD (SCD1) knockout HeLa cell line (AB265220)
Allele-2 : 4 bp deletion in exon 3.
- Sanger seq
Unknown
Sanger Sequencing - Human SCD (SCD1) knockout HeLa cell line (AB265220)
Allele-3 : 4 bp deletion in exon 3.
- Sanger seq
Unknown
Sanger Sequencing - Human SCD (SCD1) knockout HeLa cell line (AB265220)
Allele-1 : 4 bp deletion in exon 3.
- Sanger seq
Unknown
Sanger Sequencing - Human SCD (SCD1) knockout HeLa cell line (AB265220)
Allele-4 : Insertion of the selection cassette in exon 3.
- Sanger seq
Unknown
Sanger Sequencing - Human SCD (SCD1) knockout HeLa cell line (AB265220)
Allele-5 : Insertion of the selection cassette in exon 3.
Reactivity data
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SCD1 plays a significant role in fatty acid metabolism by regulating the balance of saturated and unsaturated fatty acids which impacts membrane fluidity and cellular signaling. SCD1 does not function as part of a multi-protein complex acting independently in the endoplasmic reticulum. Its expression and activity influence lipid biosynthesis and storage affecting energy homeostasis and lipid composition in cells.
Pathways
Fatty acid synthesis and oxidation pathways extensively involve SCD1. It participates mainly in the lipogenesis pathway interacting with proteins like acetyl-CoA carboxylase (ACC) and fatty acid synthase (FAS). SCD1 contributes to maintaining the ratio of oleate and palmitoleate key monounsaturated fatty acids important for cellular functions by integrating into the lipid metabolism network.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
Cancer science 112:1457-1470 PubMed33511729
2021
Applications
Unspecified application
Species
Unspecified reactive species
Complementary Products
Primary Antibodies
AB236868
KO Validated|RabMAb|Recombinant|20ul selling size
![Immunocytochemistry/ Immunofluorescence - Anti-SCD1 antibody [EPR21963] (AB236868)](https://content.abcam.com/products/images/scd1-antibody-epr21963-ab236868--immunocytochemistry-immunofluorescence-img129311.jpg)
- 0
- 0
Primary Antibodies
AB9485
Anti-GAPDH antibody - Loading Control
BOND RX™ Validated|Lab Essentials
- 5
- 88
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com