SDHAF2 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 2 bp deletion in exon 1.
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Key facts
- Cell type
HeLa
- Species or organism
Human
- Tissue
Cervix
- Form
Liquid
- Knockout validation
Sanger Sequencing
- Mutation description
Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 2 bp deletion in exon 1
Alternative names
Chromosome 11 open reading frame 79, FLJ20487, PGL2, Paraganglioma or familial glomus tumors 2, SDH assembly factor 2, SDH5, SDHF2_HUMAN, Sdhaf2, Succinate dehydrogenase assembly factor 2, mitochondrial, Succinate dehydrogenase subunit 5, hSDH5, mitochondrial
Recommended products
SDHAF2 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 2 bp deletion in exon 1.
Key facts
- Cell type
HeLa
- Species or organism
Human
- Tissue
Cervix
- Form
Liquid
- Knockout validation
Sanger Sequencing
- Mutation description
Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 2 bp deletion in exon 1
- Disease
Adenocarcinoma
- Concentration
Properties
- Gene name
SDHAF2
- Gene editing type
Knockout
- Gene editing method
CRISPR technology
- Knockout validation
Sanger Sequencing
Quality control
- STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
EU: 2 US: 2
- Adherent/suspension
Adherent
- Gender
Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
Dry Ice
- Appropriate short-term storage conditions
-196°C
- Appropriate long-term storage conditions
-196°C
Notes
Recommended control: Human wild-type HeLa cell line (Human wild-type HeLa cell line ab255928). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
C11orf79 also known as HAPSTR1 is a protein coding gene. It has a molecular mass of approximately 19 kDa. Researchers have detected its expression in various tissues including the bone marrow spleen and thymus suggesting its physiological role in immune-related contexts. C11orf79 acts mechanistically in cellular contexts where it may influence protein synthesis or transport although detailed mechanical functions remain under investigation.
- Biological function summary
C11orf79 impacts cellular processes in the immune system and has links to the HAPSTR complex where it interacts with other proteins to regulate hematopoiesis. The protein contributes to maintaining normal cell function by aiding in the regulation of proliferation and differentiation of myeloid and lymphoid cells. This suggests its involvement in general immune response and hematopoietic homeostasis.
- Pathways
C11orf79 participates in pathways related to immune cell development and function. Notably it engages in the Notch signaling pathway which influences hematopoietic cell differentiation and lymphocyte development. Additionally it interacts with proteins such as NOTCH1 and RBPJ which are essential in both the regulation of developmental processes and maintaining cellular identity.
- Associated diseases and disorders
C11orf79 has associations with immune-related diseases like autoimmune lymphoproliferative syndrome (ALPS) and certain leukemias. Its dysfunction may disrupt normal immune cell regulation exacerbating these conditions. Additionally this gene has interactions with proteins like FAS and BAG5 which can influence cell apoptosis and survival suggesting a role in disease pathogenesis where apoptotic pathways become impaired.
Product promise
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2 product images
Sanger Sequencing - Human SDHAF2 (C11orf79) knockout HeLa cell line (ab265764)
Allele-1: 2 bp deletion in exon 1.
Sanger Sequencing - Human SDHAF2 (C11orf79) knockout HeLa cell line (ab265764)
Allele-2: 1 bp deletion in exon 1.
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