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SELENBP1 KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon 2 and 1 bp insertion in exon 2.

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Images

Western blot - Human SELENBP1 (Selenium Binding Protein 1/SBP) knockout HeLa cell line (AB265279), expandable thumbnail
  • Sanger Sequencing - Human SELENBP1 (Selenium Binding Protein 1/SBP) knockout HeLa cell line (AB265279), expandable thumbnail
  • Sanger Sequencing - Human SELENBP1 (Selenium Binding Protein 1/SBP) knockout HeLa cell line (AB265279), expandable thumbnail

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Form

Liquid

Knockout validation

Sanger Sequencing, Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon 2 and 1 bp insertion in exon 2

Alternative names

56 kDa selenium-binding protein, LPSB, SBP, SBP1_HUMAN, SBP56, SELNBP1, SP56, Selenbp1, Selenbp2, Selenium binding protein 2, Selenium-binding protein 1, hSBP

Recommended products

SELENBP1 KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon 2 and 1 bp insertion in exon 2.

Key facts

Cell type

HeLa

Form

Liquid

Mutation description

Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon 2 and 1 bp insertion in exon 2

Disease

Adenocarcinoma

Concentration
Loading...

Properties

Gene name

SELENBP1

Gene editing type

Knockout

Gene editing method

CRISPR technology

Knockout validation

Sanger Sequencing, Western blot

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

Handling procedures

Initial handling guidelines

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.

Subculture guidelines

  • All seeding densities should be based on cell counts gained by established methods.

  • A guide seeding density of 2x104 cells/cm2 is recommended.

  • Cells should be passaged when they have achieved 80-90% confluence.

Culture medium

DMEM (High Glucose) + 10% FBS

Cryopreservation medium

Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-196°C

Appropriate long-term storage conditions

-196°C

Notes

Recommended control: Human wild-type HeLa cell line (Human wild-type HeLa cell line ab255928). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.

We will provide viable cells that proliferate on revival.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Selenium Binding Protein 1 (SBP1) also known as SELENBP1 is a protein of molecular weight approximately 56 kDa. It is involved in various cellular mechanisms. SBP1 binds selenium thereby playing a role in selenium metabolism and regulation within the cell. It is expressed in numerous tissues including liver kidney and colon indicating a broad physiological significance. Its expression is subject to tight regulation pointing to its importance in cellular functions.

Biological function summary

Selenium Binding Protein 1 interacts with selenium implicating it in various metabolic processes. It does not act as part of a larger complex but functions independently to influence cellular responses to selenium levels. Research shows that SBP1 plays a role in antioxidant defense and cellular detoxification suggesting a protective function against oxidative stress. This regulation supports various metabolic pathways by modulating selenium-dependent enzyme activity.

Pathways

Selenium Binding Protein 1 has been connected to the antioxidant defense pathway and the detoxification process. It interacts with proteins involved in these pathways such as glutathione peroxidases essential for eliminating oxidative damage in cells. By influencing these pathways SBP1 helps maintain cellular homeostasis highlighting its role in the broader metabolic network.

Associated diseases and disorders

Aberrations in Selenium Binding Protein 1 levels are linked to certain cancers and neurological disorders. Studies show a decrease in SBP1 expression in cancers such as colorectal cancer possibly related to reduced cellular detoxification capacity. Additionally some neurological disorders have shown altered SBP1 expression which could connect the protein with enhanced oxidative stress and cell damage. By affecting conditions like cancer SBP1 could interact with proteins involved in cell proliferation and survival supporting research on therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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