SERPINB2 KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control provided.
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Key facts
- Cell type
- U-87 MG
- Species or organism
- Human
- Tissue
- Brain
- Form
- Liquid
- Knockout validation
- Next Generation Sequencing
Recommended products
SERPINB2 KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control provided.
Key facts
- Cell type
- U-87 MG
- Species or organism
- Human
- Tissue
- Brain
- Form
- Liquid
- Knockout validation
- Next Generation Sequencing
- Disease
- Glioblastoma
- Concentration
Properties
- Gene name
- SERPINB2
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Next Generation Sequencing
Cell culture
- Biosafety level
- EU: 1 US: 1
- Viability
- ~ 80%
- Adherent/suspension
- Adherent
- Gender
- Male
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- EMEM + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
Recommended control: Human wild-type U-87 MG cell line (ab278079). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SerpinB2 also known as PAI-2 or plasminogen activator inhibitor type 2 is a serine protease inhibitor with a molecular mass of around 60 kDa. This protein localizes mostly in the cytoplasm and occasionally gets secreted outside the cell. SerpinB2 expresses in a variety of cells including monocytes macrophages and certain epithelial cells. Its primary function involves inhibiting the urokinase plasminogen activator important for processes like fibrinolysis and tissue remodeling.
- Biological function summary
SerpinB2 acts as a protector against excessive proteolysis by regulating extracellular matrix hydrolysis. While not permanently part of a complex SerpinB2 associates transiently with target proteases to modulate their activities. This regulation plays a role in cell migration and inflammation control impacting wound healing and immune responses.
- Pathways
SerpinB2 integrates into the plasminogen activation system which is important for fibrinolysis and maintaining hemostatic balance. It works closely with other serpins and proteases such as PAI-1 and urokinase-type plasminogen activator (uPA) to control the conversion of plasminogen to plasmin. This balance impacts tissue repair and may influence invasive cell behavior through the regulation of pericellular proteolysis.
- Associated diseases and disorders
SerpinB2's alteration has been linked to the development of atherosclerosis and cancer. Research shows that SerpinB2 levels can influence the inflammatory environment possibly driving atherogenic plaque development. Similarly changes in SerpinB2 expression and activity relate to cancer metastasis potentially by affecting the balance of matrix degradation and rebuilding alongside proteins like PAI-1 and uPA to modulate tumor invasiveness and growth.
Product promise
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1 product image
Next Generation Sequencing - Human SERPINB2 knockout U-87 MG cell line (ab306850)
61 bp deletion after Asn65 of the WT protein
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