Human SETD2 (KMT3A / HYPB / HIF-1) knockout HEK-293T cell line
- Advanced Validation
- What is this?
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SETD2 KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon 3 and 4 bp deletion in exon 3.
View Alternative Names
EC 2.1.1.43, FLJ16420, FLJ22472, FLJ23184, FLJ45883, HBP231, HIF-1, HSPC069, HYPB, Histone-lysine N-methyltransferase SETD2, Huntingtin interacting protein, Huntingtin interacting protein HYPB, Huntingtin yeast partner B, Huntingtin-binding protein, 231-KD, Huntingtin-interacting protein 1, Huntingtin-interacting protein B, KIAA1732, KMT3A, Lysine N-methyltransferase 3A, SET domain containing 2, SET domain-containing protein 2, SET2, SETD2_HUMAN, hSET2, p231HBP
- WB
Lab
Western blot - Human SETD2 (KMT3A / HYPB / HIF-1) knockout HEK-293T cell line (AB266690)
Lanes 1-3 : Merged signal (red and green). Green - ab31358 observed at 288 kDa. Red - loading control, ab7291 observed at 50 kDa.
ab31358 Anti-KMT3A / HYPB / HIF-1 antibody was shown to specifically react with KMT3A / HYPB / HIF-1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266690 (knockout cell lysate ab257274) was used. Wild-type and KMT3A / HYPB / HIF-1 knockout samples were subjected to SDS-PAGE. ab31358 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti- Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti- Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-KMT3A / HYPB / HIF-1 antibody (<a href='/en-us/products/primary-antibodies/kmt3a-hypb-hif-1-antibody-ab31358'>ab31358</a>) at 1/500 dilution
Lane 1:
Wild-type HEK293T cell at 20 µg
Lane 2:
Western blot - Human SETD2 (KMT3A / HYPB / HIF-1) knockout HEK-293T cell lysate (<a href='/en-us/products/cell-lysates/human-setd2-kmt3a-hypb-hif-1-knockout-hek-293t-cell-lysate-ab257274'>ab257274</a>) at 20 µg
Lane 2:
Western blot - Human SETD2 (KMT3A / HYPB / HIF-1) knockout HEK-293T cell line (ab266690)
Lane 3:
HepG2 cell lysate at 20 µg
Predicted band size: 288 kDa
Observed band size: 288 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human SETD2 (KMT3A / HYPB / HIF-1) knockout HEK-293T cell line (AB266690)
Allele-2 : 4 bp deletion in exon 3.
- Sanger seq
Lab
Sanger Sequencing - Human SETD2 (KMT3A / HYPB / HIF-1) knockout HEK-293T cell line (AB266690)
Sequencing chromatogram displaying sequence edit in exon 3
- Sanger seq
Unknown
Sanger Sequencing - Human SETD2 (KMT3A / HYPB / HIF-1) knockout HEK-293T cell line (AB266690)
Allele-1 : 11 bp deletion in exon 3
Reactivity data
Product details
Recommended control: Human wild-type HEK293T cell line (ab255449). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
KMT3A modifies chromatin structure to regulate gene expression. Its histone methylation function plays a critical role in the modulation of transcriptional activity ensuring that genes are turned on or off when necessary for cellular function. Additionally KMT3A forms part of complex protein networks that mediate gene expression changes in response to cellular signals. The precise regulation of this activity is vital for maintaining normal cellular function and responding to environmental cues.
Pathways
KMT3A engages in significant pathways such as the transcriptional regulation and epigenetic modification pathways. It interacts with various proteins including CoREST and LSD1 within these pathways to modulate gene expression and influence cellular differentiation. Through these interactions KMT3A ensures the correct genetic programs are executed aligning with developmental and homeostatic requirements.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Complementary Products
Primary Antibodies
AB239350
Anti-KMT3A / HYPB / HIF-1 antibody [EPR20927-67]
RabMAb|Recombinant|KO Validated|Trial Size
![Western blot - Anti-KMT3A / HYPB / HIF-1 antibody [EPR20927-67] (AB239350)](https://content.abcam.com/products/images/kmt3a-hypb-hif-1-antibody-epr20927-67-ab239350--western-blot-img3294.jpg)
- 0
- 0
Cell Lines & Lysates
AB255394
Human HIF1A (HIF-1 alpha) knockout HCT116 cell line
Advanced Validation
- 5
- 2
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com