SHPK KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 4.
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Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 4
Alternative names
CARKL, Carbohydrate kinase like, Carbohydrate kinase-like protein, SHPK_HUMAN, Sedoheptulokinase
Recommended products
SHPK KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 4.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 4
- Concentration
Properties
- Gene name
- SHPK
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SHK also known as Shaker-related voltage-gated potassium channel or Kv1.3 functions in the regulation of ion flow across the cell membrane. It belongs to the voltage-gated potassium channel family and has a molecular mass of approximately 57 kDa. Expression of SHK occurs mainly in the nervous system including the brain and in lymphocytes playing an important role in maintaining the cellular membrane potential and modulating action potentials.
- Biological function summary
This channel is involved in repolarizing the neuronal membrane following action potentials. SHK channels contribute significantly to the excitability of neurons and T cells facilitating their activation and response. The protein does not exist in isolation but forms part of a complex with other ion channels impacting cellular excitability and immune responses. This interaction with other ion channels allows it to fine-tune the physiological responses important for cellular signaling.
- Pathways
SHK participates in important neurological and immune system pathways. It is part of the neuronal signaling pathways where it affects neurotransmitter release. Additionally SHK aligns with the JAK-STAT pathway in T cells influencing their maturation and function. SHK operates alongside proteins like Kv1.1 in these pathways interacting to modulate channel conductance and overall cellular activity.
- Associated diseases and disorders
It is associated with multiple sclerosis and type 1 diabetes. SHK channel dysregulation affects the immune system contributing to aberrant immune responses seen in these autoimmune diseases. The protein interacts with other channels including Kv1.5 influencing disease progression. Targeting SHK for therapeutic intervention offers possible strategies for managing these conditions by correcting the dysregulated channel activity.
Product promise
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1 product image
Sanger Sequencing - Human SHPK (SHK) knockout HEK-293T cell line (ab267257)
Homozygous: 2 bp deletion in exon4
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