Human SIK1 knockout HeLa cell line
Be the first to review this product! Submit a review
|
(0 Publication)
SIK1B KO cell line available to order. KO validated by. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.
View Alternative Names
KID2, MSK, QIK, Qin-induced kinase, SIK1_HUMAN, SNF1 like kinase, Salt inducible kinase 1, Salt-inducible protein kinase 1, Serine threonine protein kinase SNF1 like kinase 1, Serine threonine protein kinase SNF1LK, Serine/threonine-protein kinase SIK1, Serine/threonine-protein kinase SNF1-like kinase 1, Serine/threonine-protein kinase SNF1LK, Snf1lk, myocardial SNF1 like kinase
- Sanger seq
Unknown
Sanger Sequencing - Human SIK1 knockout HeLa cell line (AB265681)
Homozygous : 1 bp insertion in exon 2.
Product details
Recommended control: Human wild-type HeLa cell line (ab255928). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Salt-inducible kinase 1 plays an important role in regulating cellular responses to metabolic stress and hormonal signaling. It forms part of a kinase complex where it operates alongside other kinases and regulatory proteins to modulate gene expression and metabolic pathways. SIK1 influences gluconeogenesis and lipogenesis processes essential for maintaining energy homeostasis. The regulation by SIK1 of these processes is essential to adapt to changes in cellular environment and energy requirements.
Pathways
Salt-inducible kinase 1 is an important regulator within the cAMP signaling pathway and the AMPK signaling pathway. In the cAMP pathway SIK1 interacts with protein kinase A (PKA) contributing to the modulation of the transcription factor CREB. In the AMPK pathway SIK1 indirectly affects energy balance by influencing downstream genes involved in glucose production and fatty acid metabolism. These interactions highlight SIK1's place in controlling energy and metabolic homeostasis in response to external stimuli.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB255701
Anti-SIK3 antibody [EPR23554-71]
BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated|Trial Size
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIK3 antibody [EPR23554-71] (AB255701)](https://content.abcam.com/products/images/sik3-antibody-epr23554-71-ab255701--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img164399.jpg)
- 0
- 0
Primary Antibodies
AB199474
Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196]
RabMAb|Recombinant
![Western blot - Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (AB199474)](https://content.abcam.com/products/images/sik1-phospho-t182-sik2-phospho-t175-sik3-phospho-t163-antibody-epr19196-ab199474--western-blot-img60828.jpg)
- 5
- 3
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com