Human SLC25A13 (Citrin) knockout HeLa cell line
- Advanced Validation
- What is this?
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SLC25A13 KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 3 and 1 bp insertion in exon 3.
View Alternative Names
AI785475, ARALAR2, CMC2_HUMAN, CTLN2, Calcium-binding mitochondrial carrier protein Aralar2, Citrin, Ctrn, Mitochondrial aspartate glutamate carrier 2, RGD1565889, Slc25a13, Solute carrier family 25 (citrin) member 13, Solute carrier family 25 member 13, Solute carrier family 25 member 13 (citrin)
- Sanger seq
Unknown
Sanger Sequencing - Human SLC25A13 (Citrin) knockout HeLa cell line (AB265668)
Allele-1 : 1 bp deletion in exon 3.
- Sanger seq
Unknown
Sanger Sequencing - Human SLC25A13 (Citrin) knockout HeLa cell line (AB265668)
Allele-2 : 1 bp insertion in exon 3.
Reactivity data
Product details
Recommended control: Human wild-type HeLa cell line (ab255928). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Citrin plays an important role in the urea cycle gluconeogenesis and lipid metabolism by facilitating the exchange of cytosolic glutamate for mitochondrial aspartate. It operates independently not as part of a larger protein complex. By ensuring the appropriate balance of amino acids and metabolites Citrin contributes to maintaining metabolic homeostasis. The protein's activity supports the liver's function and influences energy production efficiency especially under anabolic conditions.
Pathways
Citrin is integral to the malate-aspartate shuttle and the urea cycle. The malate-aspartate shuttle is involved in transferring reducing equivalents across the mitochondrial membrane which is essential for efficient ATP production. Citrin interacts closely with mitochondrial enzymes like carbamoyl phosphate synthetase I in the urea cycle emphasizing its link to ammonia detoxification. Citrin's transport activity indirectly supports the function of other mitochondrial carriers including SLC25A12 and SLC25A11 by maintaining the requisite balance of substrates required for their processes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Complementary Products
Primary Antibodies
AB167166
Anti-SLC25A13/Citrin antibody [EPR9969(B)]
RabMAb|Recombinant|KO Validated
![Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] (AB167166)](https://content.abcam.com/products/images/slc25a13-citrin-antibody-epr9969b-ab167166--western-blot-img1460.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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