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SLC2A3 KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control provided.

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Next Generation Sequencing - Human SLC2A3 knockout A549 cell line (AB301240), expandable thumbnail

Key facts

Cell type
A549
Species or organism
Human
Tissue
Lung
Form
Liquid
Knockout validation
Next Generation Sequencing

Alternative names

FLJ90380, GLUT-3, GTR3_HUMAN, Glucose transporter type 3, Glucose transporter type 3 brain, Slc2a3, Solute Carrier Family 2 (Facilitated Glucose Transporter) Member 3, Solute carrier family 2, facilitated glucose transporter member 3

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SLC2A3 KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control provided.

Key facts

Cell type
A549
Form
Liquid
Disease
Carcinoma
Concentration
Loading...

Properties

Gene name
SLC2A3
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Next Generation Sequencing

Cell culture

Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male

Handling procedures

Initial handling guidelines

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.

Subculture guidelines
  • All seeding densities should be based on cell counts gained by established methods.
  • A guide seeding density of 2x104 cells/cm2 is recommended.
  • Cells should be passaged when they have achieved 80-90% confluence.
  • Do not allow the cell density to exceed 7x104 cells/cm2.
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-196°C
Appropriate long-term storage conditions
-196°C

Notes

Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.

Recommended control: Human wild-type A549 cell line (ab288558). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.

We will provide viable cells that proliferate on revival.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Glucose Transporter GLUT3 also known as GLUT-3 or SLC2A3 serves as an important protein in glucose transport across cell membranes. It is part of the facilitative glucose transporter family and exhibits a molecular weight of approximately 54 kDa. This transporter shows high affinity for glucose making it particularly important in cells with high demands for glucose. You mainly find GLUT3 expressed in brain neurons placenta and sperm where it ensures sufficient glucose uptake to sustain energy-intensive activities.

Biological function summary

The transporter protein mediates the rapid uptake of glucose into cells especially under low glucose concentrations supporting high cellular metabolic rates. It does not function alone but collaborates in transporting glucose to meet cellular needs efficiently. Although GLUT3 operates as a specific transporter it is involved in a larger network that ensures glucose availability for vital cellular processes ensuring that cells like neurons maintain function even under varying glucose availability.

Pathways

The transporter plays an integral role in the glycolysis and cellular respiration pathways. It acts in concert with other glucose transporters such as GLUT1 and GLUT4 which also facilitate glucose uptake into various cells allowing efficient energy production. The proper function of glycolysis heavily depends on the effective operation of glucose transporters where GLUT3's high affinity for glucose supports metabolic activities specifically in neural cells which relies on glucose as a primary energy source.

Associated diseases and disorders

Several studies link GLUT3 to conditions like Alzheimer's disease and Type 2 diabetes. In Alzheimer's impaired glucose metabolism in the brain partly due to dysfunctional GLUT3 contributes to neurodegenerative processes. Similarly altered expression of GLUT3 affects glucose homeostasis playing a part in Type 2 diabetes pathophysiology alongside proteins like insulin receptors and other glucose transporters. These associations underline the significance of maintaining GLUT3 function for cellular glucose management and metabolic health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Next Generation Sequencing - Human SLC2A3 knockout A549 cell line (ab301240), expandable thumbnail

    Next Generation Sequencing - Human SLC2A3 knockout A549 cell line (ab301240)

    80 bp deletion after Arg 123 (allele 1); 7 bp deletion after Leu 121 (allele 2); 81 bp deletion after Arg 123 (allele 3); 8 bp deletion after Leu 121 (allele 4) of WT protein

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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