SLC30A1 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 20 bp deletion in exon 1 and 3 bp deletion in exon 1.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 20 bp deletion in exon 1 and 3 bp deletion in exon 1
Alternative names
SLC30 A1, SLC30A 1, SLC30A1, Solute carrier family 30 (zinc transporter), member 1, Solute carrier family 30 member 1, ZNT1_HUMAN, ZRC 1, Zinc transporter 1
Recommended products
SLC30A1 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 20 bp deletion in exon 1 and 3 bp deletion in exon 1.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 20 bp deletion in exon 1 and 3 bp deletion in exon 1
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- SLC30A1
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ZnT1 also known as SLC30A1 is a critical protein responsible for the export of zinc ions out of cells. This transporter plays an important role in maintaining intracellular zinc homeostasis. The protein has a molecular mass of approximately 52 kDa. ZnT1 is expressed in a wide range of tissues including the brain liver and intestines indicating its significant role in various physiological processes. The protein is located in the cell plasma membrane which aligns with its function of mediating zinc efflux.
- Biological function summary
ZnT1 is essential for regulating the cellular zinc levels and protecting against zinc toxicity. It does not form part of a larger complex; instead it operates as an individual unit to ensure that excess zinc does not accumulate to harmful levels within the cell. By controlling zinc efflux ZnT1 influences processes such as cell signaling and apoptosis which rely on zinc as a cofactor or structural component.
- Pathways
Homeostasis of zinc ions in cells connects ZnT1 to the cellular zinc transport pathway and the metal ion transport pathway. ZnT1 coordinates with intracellular zinc-binding proteins like metallothioneins to modulate zinc availability and transportation within the cell. Proteins such as ZIP4 which facilitate zinc uptake operate in a complementary manner to ZnT1 balancing zinc influx and efflux to maintain cellular function.
- Associated diseases and disorders
Misregulation of ZnT1 has been linked to conditions such as cancer and neurodegenerative diseases. In cancer changes in zinc transport influence tumor growth and progression partly involving interactions with proteins like p53 a critical tumor suppressor modulated by zinc availability. In neurodegenerative diseases abnormal zinc levels can contribute to neuronal damage where ZnT1 interacts with Amyloid Beta plaques affecting the pathology of Alzheimer's disease.
Product promise
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Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Sanger Sequencing - Human SLC30A1 (ZnT1) knockout HeLa cell line (ab264664)
Allele-1: 20 bp deletion in exon 1.
Sanger Sequencing - Human SLC30A1 (ZnT1) knockout HeLa cell line (ab264664)
Allele-2: 3 bp deletion in exon 1.
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