Human SLC38A9 knockout HeLa cell line
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SLC38A9 KO cell line available to order. KO validated. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and 5 bp deletion in exon 3. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.
View Alternative Names
Up-regulated in lung cancer 11, Solute carrier family 38 member 9, SLC38A9, Neutral amino acid transporter 9, URLC11
- Sanger seq
Unknown
Sanger Sequencing - Human SLC38A9 knockout HeLa cell line (AB265823)
Allele-2 : 1 bp insertion in exon 3.
- Sanger seq
Unknown
Sanger Sequencing - Human SLC38A9 knockout HeLa cell line (AB265823)
Allele-1 : 5 bp deletion in exon 3.
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Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This protein serves as a component of the mechanistic target of rapamycin complex 1 (mTORC1) signaling pathway. SLC38A9 directly interacts with the lysosomal surface to connect amino acid availability to mTORC1 activation. By doing so it influences cell growth and autophagy processes in response to amino acid levels. As a part of the mTORC1 complex SLC38A9 plays an important function in translating extracellular cues to intracellular responses.
Pathways
SLC38A9 is integral in the amino acid sensing mechanism that regulates the mTORC1 pathway. This pathway controls vital processes like protein synthesis and cell proliferation. The protein partners with other key players in the mTOR signaling pathway including Rag GTPases and Raptor to relay amino acid abundance to the mTORC1 complex. This relationship ensures that cellular growth processes are synchronized with available nutrition resources.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability. <p>1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.<br>2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.<br>3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.<br>4. Incubate the culture at 37°C incubator with 5% CO<sub>2</sub>. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.<br>5. Once confluent passage into an appropriate flask at a density of 2x10<sup>4</sup> cells/cm<sup>2</sup>. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.</p>
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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