SLC3A2 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 14 bp deletion in exon 6.
Images
Key facts
- Cell type
HeLa
- Species or organism
Human
- Tissue
Cervix
- Form
Liquid
- Knockout validation
Sanger Sequencing
- Mutation description
Knockout achieved by using CRISPR/Cas9, Homozygous: 14 bp deletion in exon 6
Alternative names
4F2 cell-surface antigen heavy chain, 4F2 heavy chain, 4F2 heavy chain antigen, 4F2_HUMAN, 4F2hc, 4T2HC, Antigen defined by monoclonal antibody 4F2 heavy chain, Antigen identified by monoclonal antibodies 4F2 TRA1.10 TROP4 and T43, CD98 antigen, CD98 heavy chain, CD98HC, Lymphocyte activation antigen 4F2 large subunit, MDU 1, Monoclonal antibody 44D7, NACAE, Slc3a2, Solute carrier family 3 (activators of dibasic and neutral amino acid transport) member 2, Solute carrier family 3 member 2
Recommended products
SLC3A2 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 14 bp deletion in exon 6.
Key facts
- Cell type
HeLa
- Species or organism
Human
- Tissue
Cervix
- Form
Liquid
- Knockout validation
Sanger Sequencing
- Mutation description
Knockout achieved by using CRISPR/Cas9, Homozygous: 14 bp deletion in exon 6
- Antibiotic resistance
Puromycin 1µg/mL
- Disease
Adenocarcinoma
- Concentration
Properties
- Gene name
SLC3A2
- Gene editing type
Knockout
- Gene editing method
CRISPR technology
- Knockout validation
Sanger Sequencing
- Zygosity
Homozygous
Quality control
- STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
EU: 2 US: 2
- Adherent/suspension
Adherent
- Gender
Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
Dry Ice
- Appropriate short-term storage conditions
-196°C
- Appropriate long-term storage conditions
-196°C
Notes
Recommended control: Human wild-type HeLa cell line (Human wild-type HeLa cell line ab255928). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD98 also known as CD98hc CD98 protein or LAT1/CD98 is a type II membrane protein often found in association with the heavy chains of amino acid transporters like LAT1. It has a molecular mass of approximately 85-100 kDa. CD98 is widely expressed in numerous tissues including the liver kidney and various tumors. It plays an important role in cellular processes by facilitating the transport of amino acids across plasma membranes which is essential for maintaining cellular homeostasis and proliferation.
- Biological function summary
CD98 forms a complex with light chains of the heterodimeric amino acid transporters such as LAT1 and LAT2. This interaction enhances the uptake of neutral amino acids into cells. CD98 also interacts with integrins influencing cell adhesion and migration which are critical for cellular signaling and response. Through these interactions CD98 impacts not only cell survival and growth but also immune responses and angiogenesis.
- Pathways
CD98 is a vital component of the mTOR signaling pathway influencing cell growth and proliferation. It also plays a part in the integrin signaling pathway which regulates cell adhesion and migration. In these pathways CD98 works closely with proteins like FG1 and mTORC1. These relationships enable it to modulate cellular responses to nutritional availability and mechanical cues from the environment.
- Associated diseases and disorders
CD98 expression and function have links to cancer and autoimmune diseases. High expression levels of CD98 are observed in various cancers such as lymphomas where it correlates with increased tumor growth and poor prognosis. In autoimmune disorders like rheumatoid arthritis CD98 influences immune cell activation and migration. Proteins such as CP1 and FG1 interact with CD98 contributing to disease pathology by affecting cellular and immune responses.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Western blot - Human SLC3A2 (CD98) knockout HeLa cell line (ab265708)
Lanes 1 - 5:Merged signal (red and green). Green - ab253273 observed at 80 - 90 kDa. Red - loading control Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55kDa.
ab253273 was shown to react with CD98 in wild-type HeLa cells in Western blot with loss of signal observed in SLC3A2 knockout cell line ab265708 (SLC3A2 knockout cell lysate Human SLC3A2 (CD98) knockout HeLa cell lysate ab257680). Wild-type HeLa and SLC3A2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5 % milk in TBS-T (0.1 % Tween®) before incubation with ab253273 and Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4 °C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Human SLC3A2 (CD98) knockout HeLa cell line (ab265708)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SLC3A2 (CD98) KO HeLa (SLC3A2 (CD98) knockout human cervix adenocarcinoma epithelial cell) (ab265708) cells labelling CD98 with Alexa Fluor® 647 Anti-CD98 antibody [EPR27110-42] ab314339 at 1/50 (10.0 ug/ml) dilution(Red). Confocal image showing membrane staining in parental HeLa cell line, while no staining in SLC3A2 knockout HeLa cells (ab265708). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Green). The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry/ Immunofluorescence - Human SLC3A2 (CD98) knockout HeLa cell line (ab265708)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SLC3A2 (CD98) KO HeLa (SLC3A2 (CD98) knockout human cervix adenocarcinoma epithelial cell) (ab265708) cells labelling CD98 with Alexa Fluor® 594 Anti-CD98 antibody [EPR27110-42] ab314338 at 1/50 (10.0 ug/ml) dilution(Red). Confocal image showing membrane staining in parental HeLa cell line, while no staining in SLC3A2 knockout HeLa cells (ab265708). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Green). The Nuclear counterstain was DAPI (Blue).
Sanger Sequencing - Human SLC3A2 (CD98) knockout HeLa cell line (ab265708)
Homozygous: 14 bp deletion in exon 6.
Immunocytochemistry/ Immunofluorescence - Human SLC3A2 (CD98) knockout HeLa cell line (ab265708)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SLC3A2 (CD98) KO HeLa (SLC3A2 (CD98) knockout human cervix adenocarcinoma epithelial cell) (ab265708) cells labelling CD98 with Anti-CD98 antibody [EPR27110-42] ab307587 at 1/100 (4.97 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green). Confocal image showing membrane and weak cytoplasmic staining in parental HeLa cell line.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com