SLC41A3 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.
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Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2
Alternative names
1010001P06Rik, AI480742, FLJ20473, OTTMUSP00000028993, OTTMUSP00000028994, SLC41A1 L2, SLC41A1 like 2, Solute carrier family 41, member 3
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SLC41A3 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2
- Concentration
Properties
- Gene name
- SLC41A3
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SLC41A3 also known as solute carrier family 41 member 3 functions as a magnesium transporter. This protein is part of the broader SLC41 family known for their role in magnesium homeostasis. SLC41A3 has a molecular mass of approximately 56 kDa. It is expressed in various human tissues with notable presence in the heart and skeletal muscle indicating its potential role in these areas.
- Biological function summary
SLC41A3 participates in maintaining the cellular magnesium balance essential for many cellular processes such as enzyme activation and ion channel regulation. The protein acts individually; however it may interact with other magnesium transport proteins to achieve this balance. The maintenance of magnesium levels is necessary for proper cellular function and biochemical reactions.
- Pathways
The SLC41A3 protein integrates into the magnesium homeostasis pathway which involves other proteins such as TRPM7 and CNNM2. Within these pathways SLC41A3 contributes to essential physiological processes including muscle contraction and neural activity. Its function is implicit in transport and regulation mechanisms that keep magnesium concentrations within healthy ranges.
- Associated diseases and disorders
Alterations in SLC41A3 activity can contribute to magnesium deficiency affecting neuromuscular and cardiovascular systems. Dysregulation may also associate with conditions like hypertension reflecting its involvement with disorders influenced by magnesium levels. Research on SLC41A3’s interaction with proteins such as TRPM6 offers insight into potential therapeutic targets for managing these disorders.
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1 product image
Sanger Sequencing - Human SLC41A3 knockout HEK-293T cell line (ab267256)
Homozygous: 1 bp insertion in exon2
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