SLC52A2 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 1 bp insertion in exon 2.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 1 bp insertion in exon 2
Alternative names
GPR172A, PERV-A receptor 1, Porcine endogenous retrovirus A receptor 1, Protein GPR172A, RFT3, RFT3_HUMAN, Riboflavin transporter 3, hRFT3
Recommended products
SLC52A2 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 1 bp insertion in exon 2.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 1 bp insertion in exon 2
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- SLC52A2
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GPR172A also known as PAR1 is a G protein-coupled receptor with roles in various cellular processes. Its molecular mass is approximately 48 kDa. This protein is expressed in many tissues including the liver kidney and brain. As a receptor it interacts with G proteins to transmit signals from the outside of the cell to the inside influencing the cell's response to external stimuli.
- Biological function summary
GPR172A/PAR1 plays an important role in regulating blood clotting and inflammation. It is part of a signaling complex that includes thrombin a serine protease. This complex is important for activating platelets which are cells involved in the blood clotting process. By activating platelets GPR172A/PAR1 helps control bleeding and wound healing.
- Pathways
The protein is involved in both the coagulation and inflammatory pathways. In the coagulation pathway it works alongside other proteins like thrombin and fibrinogen contributing to clot formation. In inflammation GPR172A/PAR1 interacts with proteins such as NF-kB helping to regulate inflammatory responses and cytokine production which are important for immune defense and tissue repair.
- Associated diseases and disorders
GPR172A/PAR1 has a connection to cardiovascular diseases and inflammatory disorders. Its role in promoting blood clot formation links it to conditions like thrombosis where abnormal clotting can lead to dangerous blockages in blood vessels. GPR172A/PAR1 also relates to inflammatory disorders through its interaction with inflammatory proteins such as NF-kB. Abnormal activity of this receptor can lead to excessive inflammation contributing to diseases like rheumatoid arthritis.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Sanger Sequencing - Human SLC52A2 (GPR172A/PAR1) knockout HeLa cell line (ab265411)
Allele-1: 1 bp deletion in exon 2.
Sanger Sequencing - Human SLC52A2 (GPR172A/PAR1) knockout HeLa cell line (ab265411)
Allele-2: 1 bp insertion in exon 2.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com