Human SLC5A6 knockout HEK-293T cell line
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SLC5A6 KO cell line available to order. KO validated by. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 3 and 7 bp deletion in exon 3.
View Alternative Names
E430023I20, MGC109689, Na(+)-dependent multivitamin transporter, SC5A6_HUMAN, SMVT, Sodium-dependent multivitamin transporter, Solute carrier family 5 (sodium dependent vitamin transporter) member, Solute carrier family 5 member 6
- Sanger seq
Unknown
Sanger Sequencing - Human SLC5A6 knockout HEK-293T cell line (AB266804)
Allele-1 : 7 bp deletion in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human SLC5A6 knockout HEK-293T cell line (AB266804)
Allele-2 : 1 bp deletion in exon 3.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Human SLC5A6 knockout HEK-293T cell line (AB266804)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized SLC5A6 KO HEK-293T (SLC5A6 knockout human embryonic kidney epithelial cell), ab266804; Wild type HEK-293T (human embryonic kidney epithelial cell), ab255449 cells labelling SLC5A6 with ab325124 at 1/500 (1.028 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing cytoplasmic and membranous staining in wild type HEK-293T cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 (1 ug/ml) dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 (2 ug/ml) dilution (Magenta).
Product details
Recommended control: Human wild-type HEK293T cell line (ab255449). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SLC5A6 is essential for the efficient transport of vitamins across cell membranes using sodium gradient as the driving force. It transports vitamins into cells where they participate as coenzymes in several enzymatic reactions. This transporter does not form part of a larger protein complex but functions individually to link dietary intake with cellular nutrient requirements. The absorption through SLC5A6 impacts various biosynthetic processes as biotin and pantothenate contribute to fatty acid synthesis and energy metabolism.
Pathways
The role of SLC5A6 in nutrient absorption connects it to critical metabolic pathways such as the biotin and CoA biosynthetic pathways. Its function impacts cellular metabolism by providing essential cofactors for carboxylation reactions and energy production. SLC5A6 interacts with enzymes that rely on these vitamin-derived cofactors maintaining cellular metabolic equilibrium essential for cell growth and energy balance.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Complementary Products
Primary Antibodies
AB321194
Alexa Fluor® 750 Anti-Gephyrin antibody [EPR12651(B)]
Recombinant|RabMAb
- 0
- 0
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com