SLC7A1 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and 35 bp deletion in exon 3.
Images
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and 35 bp deletion in exon 3
Alternative names
ATRC1, Amino acid transporter cationic 1, CTR1_HUMAN, ERR, Ecotropic retroviral leukemia receptor homolog, Ecotropic retroviral receptor, Ecotropic retrovirus receptor homolog, HCAT1, High-affinity cationic amino acid transporter-1, REC1L, Slc7a1, Solute carrier family 7 (cationic amino acid transporter, y+ system) member 1, Solute carrier family 7 member 1, System Y+ basic amino acid transporter
Recommended products
SLC7A1 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and 35 bp deletion in exon 3.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and 35 bp deletion in exon 3
- Concentration
Properties
- Gene name
- SLC7A1
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CAT1 also known as cationic amino acid transporter 1 or SLC7A1 is an integral membrane protein that facilitates the uptake of cationic amino acids such as arginine lysine and ornithine. CAT1 is a member of the solute carrier family 7 and has a molecular mass of approximately 68 kDa. It expresses widely in various tissues with particularly high levels in the liver lung and skeletal muscle. As a transporter CAT1 plays an essential role in amino acid homeostasis by mediating amino acid transport across the cell membrane.
- Biological function summary
CAT1 influences nitric oxide production by regulating arginine availability a substrate for nitric oxide synthase. By controlling amino acid imports it affects cellular signaling and metabolism. CAT1 does not function as part of a larger complex but interacts with other transporters to maintain cellular amino acid balance. CAT1's role extends to impacting cell proliferation as cationic amino acids are important for growing tissues.
- Pathways
CAT1 has been implicated in the nitric oxide synthesis pathway where it contributes by sustaining intracellular arginine levels. This involvement is critical to vascular function and immune response regulation. CAT1 also participates in mTOR signaling by providing necessary amino acids like arginine which influence protein synthesis and cellular growth. In these pathways CAT1 connects with proteins such as mTOR and nitric oxide synthase assisting in integral cellular functions.
- Associated diseases and disorders
CAT1 links to cardiovascular conditions through its role in nitric oxide production which affects blood pressure regulation and endothelial function. Dysfunction or altered expression of CAT1 can lead to cardiovascular abnormalities due to impaired nitric oxide synthesis. CAT1 is also implicated in cancer as its impact on arginine availability affects cell proliferation and tumor growth. In this context CAT1 interacts with oncogenes and tumor suppressor proteins that modulate growth-related pathways.
Product promise
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Terms & Conditions.
2 product images
Sanger Sequencing - Human SLC7A1 (CAT1) knockout HEK-293T cell line (ab266234)
Allele-2: 1 bp insertion in exon 3.
Sanger Sequencing - Human SLC7A1 (CAT1) knockout HEK-293T cell line (ab266234)
Allele-1: 35 bp deletion in exon3
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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