Human SLC7A1 (CAT1) knockout HEK-293T cell line
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SLC7A1 KO cell line available to order. KO validated. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and 35 bp deletion in exon 3. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.
View Alternative Names
ATRC1, Amino acid transporter cationic 1, CTR1_HUMAN, ERR, Ecotropic retroviral leukemia receptor homolog, Ecotropic retroviral receptor, Ecotropic retrovirus receptor homolog, HCAT1, High-affinity cationic amino acid transporter-1, REC1L, Slc7a1, Solute carrier family 7 (cationic amino acid transporter, y+ system) member 1, Solute carrier family 7 member 1, System Y+ basic amino acid transporter
- Sanger seq
Unknown
Sanger Sequencing - Human SLC7A1 (CAT1) knockout HEK-293T cell line (AB266234)
Allele-2 : 1 bp insertion in exon 3.
- Sanger seq
Unknown
Sanger Sequencing - Human SLC7A1 (CAT1) knockout HEK-293T cell line (AB266234)
Allele-1 : 35 bp deletion in exon3
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CAT1 influences nitric oxide production by regulating arginine availability a substrate for nitric oxide synthase. By controlling amino acid imports it affects cellular signaling and metabolism. CAT1 does not function as part of a larger complex but interacts with other transporters to maintain cellular amino acid balance. CAT1's role extends to impacting cell proliferation as cationic amino acids are important for growing tissues.
Pathways
CAT1 has been implicated in the nitric oxide synthesis pathway where it contributes by sustaining intracellular arginine levels. This involvement is critical to vascular function and immune response regulation. CAT1 also participates in mTOR signaling by providing necessary amino acids like arginine which influence protein synthesis and cellular growth. In these pathways CAT1 connects with proteins such as mTOR and nitric oxide synthase assisting in integral cellular functions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com