SLCO4A1 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 1 bp insertion in exon 2 and Insertion of the selection cassette in exon 2.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 1 bp insertion in exon 2 and Insertion of the selection cassette in exon 2
Alternative names
Colon organic anion transporter, OATP-E, OATP-RP1, OATP1, OATP4A1, Organic anion transporter polypeptide-related protein 1, Organic anion-transporting polypeptide E, POAT, SLC21A12, SO4A1_HUMAN, Sodium-independent organic anion transporter E, Solute carrier family 21 (organic anion transporter) member 12, Solute carrier family 21 member 12, Solute carrier organic anion transporter family member 4A1
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SLCO4A1 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 1 bp insertion in exon 2 and Insertion of the selection cassette in exon 2.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 1 bp insertion in exon 2 and Insertion of the selection cassette in exon 2
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- SLCO4A1
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SLCO4A1 also known as solute carrier organic anion transporter family member 4A1 functions as a transporter protein involved in the translocation of organic anions across cell membranes. With an approximate mass of 80 kDa SLCO4A1 facilitates the movement of large often negatively charged molecules playing a part in cellular uptake processes. Its expression is noticeable in several tissues including the liver kidney and heart indicating its role in the systemic circulation and excretion of various substrates.
- Biological function summary
SLCO4A1 is engaged in the transport of hormones drugs and metabolites across cellular compartments. It belongs to the larger family of solute carriers and does not necessarily form part of a multi-protein complex operating as a standalone transporter. Its activity influences the cellular internalization of various biologically active compounds marking its importance in balancing intracellular and extracellular concentrations.
- Pathways
SLCO4A1 integration is seen in critical pathways like drug metabolism and steroid hormone metabolism. In the context of drug metabolism it works alongside cytochrome P450 enzymes to ensure the proper processing and elimination of pharmaceutical compounds. In steroid hormone metabolism it interacts with proteins such as SHBG (sex hormone-binding globulin) to modulate hormone bioavailability influencing physiological responses.
- Associated diseases and disorders
SLCO4A1 shows connections to conditions such as cholestasis and certain drug-induced toxicities. In cholestasis the impaired function of SLCO4A1 can disrupt bile acid transport aggravating liver function issues. Furthermore its interplay with hepatic transporters like SLCO1B1 can affect drug pharmacokinetics raising susceptibility to drug-induced liver injury. Understanding these interactions aids in assessing risk factors associated with SLCO4A1 dysfunction in disease states.
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3 product images
Sanger Sequencing - Human SLCO4A1 knockout HeLa cell line (ab265807)
Allele-2: 1 bp insertion in exon 2.
Sanger Sequencing - Human SLCO4A1 knockout HeLa cell line (ab265807)
Allele-1: 1 bp deletion in exon 2.
Sanger Sequencing - Human SLCO4A1 knockout HeLa cell line (ab265807)
Allele-3: Insertion of the selection cassette in exon 2.
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