Human SLIT1 knockout Jurkat cell line
- KO Validated
- Advanced Validation
- What is this?
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SLIT1 KO cell line available to order. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control available. Deletion. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.
View Alternative Names
MEGF4, Slit-1, SLIL1, Multiple EGF-like domains protein 4, SLIT1, Slit homolog 1 protein, Multiple epidermal growth factor-like domains protein 4, KIAA0813
- WB
Supplier Data
Western blot - Human SLIT1 knockout Jurkat cell line (AB326615)
ab151724 was shown to react with SLIT1 in wild-type Jurkat cells in Western blot with loss of signal observed in SLIT1 knockout cell line ab326615. Wild-type Jurkat and SLIT1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab151724 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.
This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
All lanes:
Western blot - Anti-Slit1 antibody [EP5797(2)] (<a href='/en-us/products/primary-antibodies/slit1-antibody-ep57972-ab151724'>ab151724</a>) at 1/1000 dilution
Lane 1:
Wild-type Jurkat lysate at 30 µg
Lane 2:
SLIT1 knock-out Jurkat lysate at 30 µg
Lane 2:
Western blot - Human SLIT1 knockout Jurkat cell line (ab326615)
Predicted band size: 167 kDa
false
- WB
Supplier Data
Western blot - Human SLIT1 knockout Jurkat cell line (AB326615)
ab183704 was shown to react with SLIT1 in wild-type Jurkat cells in Western blot with loss of signal observed in SLIT1 knockout cell line ab326616. Wild-type Jurkat and SLIT1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab183704 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.
This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
All lanes:
Western blot - Anti-Slit1 + Slit2 antibody [EP5796(N)] (<a href='/en-us/products/primary-antibodies/slit1-slit2-antibody-ep5796n-ab183704'>ab183704</a>) at 1/1000 dilution
Lane 1:
Wild-type Jurkat lysate at 30 µg
Lane 2:
SLIT1 knock-out Jurkat lysate at 30 µg
Lane 2:
Western blot - Human SLIT1 knockout Jurkat cell line (ab326615)
Predicted band size: 167 kDa
false
- NGS
Lab
Next Generation Sequencing - Human SLIT1 knockout Jurkat cell line (AB326615)
55bp deletion after His 216, exon 8 CCDS7453.1
- NGS
Lab
Next Generation Sequencing - Human SLIT1 knockout Jurkat cell line (AB326615)
allele 1- 68bp deletion after Pro 234, allele 2 - 62bp deletion Thr 235, exon 8 CCDS7453.1
Reactivity data
Product details
Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Suspension
Gender
Male
Viability
~ 60%
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability. <p>1. Thaw the vial in 37°C water for bath approximately 1-2 minutes. <br>2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution. <br>3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method. Based on cell count, seed cells in an appropriate cell culture flask at a density of 2x10<sup>5</sup> cells/mL. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. <br>4. Incubate the culture at 37°C incubator with 5% CO<sub>2</sub>. Cultures should be monitored daily.</p>
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x105 cells/mL is recommended.
- Do not allow cell density to exceed 3x106 cells/mL.
Culture medium
RPMI + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com