Human SLIT2 knockout HEK-293 cell line
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SLIT2 KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control provided. Knockout achieved by CRISPR/Cas9; X = 13 bp deletion; Frameshift: 100%.
View Alternative Names
Drad 1, E030015M03Rik, E130320P19Rik, FLJ14420, OTTHUMP00000158695, OTTHUMP00000217852, OTTHUMP00000217853, OTTHUMP00000217854, SLIT2_HUMAN, Slil 3, Slit homolog 2, Slit homolog 2 (Drosophila), Slit homolog 2 protein, Slit homolog 2 protein C-product
- NGS
Supplier Data
Next Generation Sequencing - Human SLIT2 knockout HEK-293 cell line (AB273723)
Knockout achieved by CRISPR/Cas9; X = 13 bp deletion; Frameshift : 100%
Reactivity data
Product details
Recommended control: Human wild-type HEK-293 cell line (ab259776). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Slit2 functions in guiding axons during neuronal development and preventing them from straying from their pathways. It complexes with Robo receptors to transmit repulsive directional signals. This interaction is essential in the development of the nervous system ensuring proper neural circuit formation and synaptic connectivity. Besides neural guidance Slit2 also regulates other cellular events like angiogenesis and immune cell migration.
Pathways
Slit2 participates in the axon guidance pathway where it influences neural network structuring by interacting with Robo receptors and other guidance cues. The Slit-Robo pathway plays an important role in axonal repulsion working alongside the Netrin signaling pathway that involves proteins like DCC and UNC5. This way Slit2 and its pathways balance attractive and repulsive cues to form functional neuronal networks.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Complementary Products
Primary Antibodies
AB32072
Anti-c-Myc antibody [Y69] - ChIP Grade
BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc antibody [Y69] - ChIP Grade (AB32072)](https://content.abcam.com/products/images/c-myc-antibody-y69-chip-grade-ab32072--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img80839.jpg)
- 4
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![Western blot - Anti-Slit2 antibody [EPR2771] (AB134166)](https://content.abcam.com/products/images/slit2-antibody-epr2771-ab134166--western-blot-img419317.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com