Human SLPI knockout HeLa cell line
- Advanced Validation
- What is this?
Be the first to review this product! Submit a review
|
(0 Publication)
SLPI KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.
View Alternative Names
WAP4, Seminal proteinase inhibitor, WFDC4, Mucus proteinase inhibitor, BLPI, Secretory leukocyte protease inhibitor, MPI, SLPI, Antileukoproteinase, HUSI-1, Protease inhibitor WAP4, ALP, WAP four-disulfide core domain protein 4
- WB
Lab
Western blot - Human SLPI knockout HeLa cell line (AB265215)
False colour image of Western blot : Anti-SLPI antibody - Aminoterminal end staining at 1/1000 dilution shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution shown in red. In Western blot ab46763 was shown to bind specifically to SLPI. A band was observed at 22 kDa in wild-type HeLa cell lysates with no signal observed at this size in SLPI knockout cell line ab265215 (knockout cell lysate ab257685). To generate this image wild-type and SLPI knockout HeLa cell lysates were analysed. First samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °. Blots were washed four times in TBS-T incubated with secondary antibodies for 1 h at room temperature washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye®800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-SLPI antibody - Aminoterminal end (<a href='/en-us/products/primary-antibodies/slpi-antibody-aminoterminal-end-ab46763'>ab46763</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
SLPI knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human SLPI knockout HeLa cell line (ab265215)
Lane 2:
Western blot - Human SLPI knockout HeLa cell lysate (<a href='/en-us/products/cell-lysates/human-slpi-knockout-hela-cell-lysate-ab257685'>ab257685</a>)
Lane 3:
PC-3 cell lysate at 20 µg
Lane 4:
LNCaP cell lysate at 20 µg
Predicted band size: 14 kDa
Observed band size: 22 kDa
false
- Sanger seq
Lab
Sanger Sequencing - Human SLPI knockout HeLa cell line (AB265215)
Homozygous : Insertion of the selection cassette in exon 1.
Complementary Products
![Immunocytochemistry/ Immunofluorescence - Anti-PDK1 antibody [EPR19571] (AB202468)](https://content.abcam.com/products/images/pdk1-antibody-epr19571-ab202468--immunocytochemistry-immunofluorescence-img110963.jpg)
Primary Antibodies
AB231828
Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free
RabMAb|Recombinant
![Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (AB231828)](https://content.abcam.com/products/images/glucose-6-phosphate-dehydrogenase-antibody-epr20668-bsa-and-azide-free-ab231828--immunocytochemistry-immunofluorescence-img73529.jpg)
- 4
- 6
Cell Lines & Lysates
AB266899
Human UBE2K (HIP2/LIG) knockout HCT116 cell line
Advanced Validation
- 0
- 0
Reactivity data
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SLPI serves as a protector of epithelial surfaces from proteolytic degradation and acts as an anti-inflammatory agent. It displays broad-spectrum antimicrobial properties providing protection against bacterial and viral infections. Although SLPI is not part of a complex itself its interactions with extracellular matrix proteins help modulate wound healing processes and maintain tissue integrity.
Pathways
SLPI participates in the innate immune response and inflammation regulation pathways. It interacts within pathways that involve the complement cascade and kallikrein-kinin system helping to regulate inflammation and modulate immune responses. During these processes SLPI is related to proteins such as alpha-1-antitrypsin in its role as a protease inhibitor contributing to the regulation of protease activity and inflammatory responses.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability. <p>1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.<br>2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.<br>3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.<br>4. Incubate the culture at 37°C incubator with 5% CO<sub>2</sub>. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.<br>5. Once confluent passage into an appropriate flask at a density of 2x10<sup>4</sup> cells/cm<sup>2</sup>. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.</p>
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com