SMARCAL1 KO cell line available now. Free of charge wild type control available.
PC-3
Human
Prostate
Liquid
HARP, HepA-related protein, SIOD, SMAL1_HUMAN, SMARCA like Protein 1, SWI/SNF-related, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1, Sucrose nonfermenting protein 2-like 1, hHARP, smarcal1
SMARCAL1 KO cell line available now. Free of charge wild type control available.
HARP, HepA-related protein, SIOD, SMAL1_HUMAN, SMARCA like Protein 1, SWI/SNF-related, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1, Sucrose nonfermenting protein 2-like 1, hHARP, smarcal1
PC-3
Human
Prostate
Liquid
Adenocarcinoma
SMARCAL1
Knockout
CRISPR technology
EU: 1 US: 1
Adherent
Male
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
F-12K + 10% FBS
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Dry Ice
-196°C
Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.
Recommended control: Human wild-type PC-3 cell line (Human wild-type PC-3 cell line ab290718). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
SmarcAL1 also known as SWI/SNF-related ATPase family member A1 is a DNA-dependent ATPase that unwinds DNA during replication and repair. It also plays a role in chromatin remodeling. The molecular mass of SmarcAL1 is approximately 105 kDa. SmarcAL1 expression is particularly noticeable in tissues with high replicative demands like testis and ovary among others.
SmarcAL1 facilitates proper replication fork stability and functioning by associating with other proteins and complexes. It is a part of the SWI/SNF chromatin remodeling complex which alters the structure of chromatin to regulate access to DNA. This protein works with other chromatin remodeling factors to ensure the DNA replication and repair processes run smoothly.
SmarcAL1 is a significant player in DNA damage response and repair pathways. It is actively involved in the homologous recombination repair pathway. Associated proteins within this pathway include BRCA1 and RAD51 which are essential for repairing double-strand breaks in DNA. Together these proteins promote genomic stability by correcting DNA damage and supporting normal cell cycle progression.
Mutations or dysfunctions in SmarcAL1 have connections to Schimke immuno-osseous dysplasia (SIOD) and various cancer types. These associations arise because SmarcAL1 influences genome stability and cell cycle progression. The connection of SmarcAL1 with BRCA1 highlights its relevance in breast cancer where malfunction in DNA repair mechanisms contribute to cancer development.
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Terms & Conditions.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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