Human SMARCC1 (BAF155) knockout HEK-293 cell line
- Advanced Validation
- What is this?
Be the first to review this product! Submit a review
|
(0 Publication)
- WB
Lab
Western blot - Human SMARCC1 (BAF155) knockout HEK-293 cell line (AB261854)
Lanes 1 - 4 : Merged signal (red and green). Green - ab172636 observed at 123 kDa. Red - loading control ab8245 observed at 37 kDa.
ab172636 was shown to specifically react with SMARCC1 in wild-type HEK-293 cells as signal was lost in SMARCC1 knockout cells. Wild-type and SMARCC1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab172636 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4° at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-SMARCC1/BAF155 antibody [EPR12389] (<a href='/en-us/products/primary-antibodies/smarcc1-baf155-antibody-epr12389-ab172636'>ab172636</a>) at 1/5000 dilution
Lane 1:
Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
Lane 2:
SMARCC1 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
Lane 2:
Western blot - Human SMARCC1 (BAF155) knockout HEK-293 cell line (ab261854)
Lane 3:
HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 4:
Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg
Predicted band size: 123 kDa
Observed band size: 123 kDa
false
- WB
Lab
Western blot - Human SMARCC1 (BAF155) knockout HEK-293 cell line (AB261854)
Lanes 1 - 4 : Merged signal (red and green). Green - ab172638 observed at 123 kDa. Red - loading control ab8245 observed at 37 kDa.
ab172638 was shown to recognize in wild-type HEK293 cells as signal was lost at the expected MW in SMARCC1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and SMARCC1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab172638 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4° at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-SMARCC1/BAF155 antibody [EPR12395] - ChIP Grade (<a href='/en-us/products/primary-antibodies/smarcc1-baf155-antibody-epr12395-chip-grade-ab172638'>ab172638</a>) at 1/5000 dilution
Lane 1:
Wild-type HEK293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
Lane 2:
SMARCC1 knockout HEK293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
Lane 2:
Western blot - Human SMARCC1 (BAF155) knockout HEK-293 cell line (ab261854)
Lane 3:
HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 4:
Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg
Predicted band size: 123 kDa
false
- NGS
Supplier Data
Next Generation Sequencing - Human SMARCC1 (BAF155) knockout HEK-293 cell line (AB261854)
2 bp insertion (allele 1), 1 bp insertion and 1 bp deletion (allele 2), and 1 bp insertion (allele 3) after Asp174 of the WT protein
Complementary Products
Cell Lines & Lysates
AB265069
Human FOXO3 (FOXO3A) knockout HEK-293T cell line
Advanced Validation
- 0
- 0
Primary Antibodies
AB305038
Anti-SMARCC1/BAF155 antibody [EPR25109-77] - BSA and Azide free
BOND RX™ Validated|RabMAb|Recombinant|KO Validated
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCC1/BAF155 antibody [EPR25109-77] - BSA and Azide free (AB305038)](https://content.abcam.com/products/images/smarcc1-baf155-antibody-epr25109-77-bsa-and-azide-free-ab305038--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img403516.jpg)
- 0
- 0
Reactivity data
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SMARCC1 participates in remodeling chromatin architecture to facilitate or repress gene transcription. It forms an integral part of the SWI/SNF complex which is critical for maintaining proper chromatin structure and function. This complex influences gene expression during developmental processes cell cycle regulation and DNA repair. SMARCC1 interacts with other core components of the SWI/SNF complex such as BAF47 and BRG1 ensuring correct and responsive chromatin modification.
Pathways
SMARCC1 participates in the ATP-dependent chromatin remodeling pathway an important player in gene expression control. This remodeling is connected to the cell cycle pathway as it regulates genes required for cell proliferation. Within these pathways SMARCC1 closely interacts with transcription factors such as c-Myc and other chromatin regulators coordinating the expression of target genes necessary for cell growth and differentiation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com