Human SNAI1 (SNAIL) knockout HeLa cell line
- Advanced Validation
- What is this?
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SNAI1 KO cell line available to order. KO validated by. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 5 bp deletion in exon 2 and 68 bp insertion in exon 2.
View Alternative Names
Protein sna, Protein snail homolog, Protein snail homolog 1, SLUGH2, SNA, SNAH, SNAI, SNAI1_HUMAN, SNAIL, SNAIL, Drosophila, homolog of, 1, SNAIL1, Sna protein, Snail 1 homolog, Snail 1 zinc finger protein, Snail family transcriptional repressor 1, Snail homolog 1 (Drosophila), Zinc finger protein SNAI1, dJ710H13.1
- WB
Lab
Western blot - Human SNAI1 (SNAIL) knockout HeLa cell line (AB265963)
False colour image of Western blot : Anti-SNAIL antibody [EPR21043] staining at 1/500 dilution shown in green; loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) staining at 1/20000 dilution shown in red. In Western blot ab216347 was shown to bind specifically to SNAIL. A band was observed at 33 kDa in wild-type HeLa cell lysates with no signal observed at this size in SNAI1 CRISPR-Cas9 edited cell line ab265963 (CRISPR-Cas9 edited cell lysate ab257692). The band observed in the CRISPR-Cas9 edited lysate lane below 33 kDa is likely to represent a truncated form of SNAIL. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image wild-type and SNAI1 CRISPR-Cas9 edited HeLa cell lysates were analysed. First samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °. Blots were washed four times in TBS-T incubated with secondary antibodies for 1 h at room temperature washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-SNAIL antibody [EPR21043] (<a href='/en-us/products/primary-antibodies/snail-antibody-epr21043-ab216347'>ab216347</a>) at 1/500 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
SNAI1 CRISPR-Cas9 edited HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human SNAI1 (SNAIL) knockout HeLa cell line (ab265963)
Predicted band size: 29 kDa
Observed band size: 33 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human SNAI1 (SNAIL) knockout HeLa cell line (AB265963)
Allele-1 : 5 bp deletion in exon 2.
- Sanger seq
Unknown
Sanger Sequencing - Human SNAI1 (SNAIL) knockout HeLa cell line (AB265963)
Allele-2 : 68 bp insertion in exon 2.
Reactivity data
Product details
Recommended control: Human wild-type HeLa cell line (ab255928). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SNAIL contributes to the epithelial-mesenchymal transition (EMT) a process critical for embryogenesis and tumor progression. It forms a part of a complex network of transcription factors that regulate cell-cell adhesion molecules like E-cadherin. SNAIL's ability to bind to specific DNA sequences allows it to suppress or activate the transcription of target genes promoting cellular metamorphosis and enabling cells to acquire motility and invade other tissues.
Pathways
SNAIL operates significantly within the TGF-beta and Wnt signaling pathways. The TGF-beta pathway enhances the expression of SNAIL which in turn represses genes that maintain the epithelial phenotype. The Wnt pathway also modulates SNAIL activity connecting it with proteins such as beta-catenin to drive EMT. These pathways highlight SNAIL's involvement in sophisticated signaling pathways that determine cell behavior adaptation and tissue remodeling.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Complementary Products
Primary Antibodies
AB216347
Anti-SNAIL antibody [EPR21043]
20ul selling size|KO Validated|RabMAb|Recombinant
![Western blot - Anti-SNAIL antibody [EPR21043] (AB216347)](https://content.abcam.com/products/images/snail-antibody-epr21043-ab216347--western-blot-img137742.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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