SNAPIN KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 29 bp deletion in exon 1 and 35 bp deletion in exon 1 and 50 bp deletion in exon 1 and Insertion of the selection cassette in exon 1.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 29 bp deletion in exon 1 and 35 bp deletion in exon 1 and 50 bp deletion in exon 1 and Insertion of the selection cassette in exon 1
Alternative names
OTTHUMP00000035157, SNAP-25-binding protein, SNAP-associated protein, SNAP25BP, SNAPAP, SNAPN_HUMAN, SNARE-associated protein Snapin, Synaptosomal-associated protein 25-binding protein
Recommended products
SNAPIN KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 29 bp deletion in exon 1 and 35 bp deletion in exon 1 and 50 bp deletion in exon 1 and Insertion of the selection cassette in exon 1.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 29 bp deletion in exon 1 and 35 bp deletion in exon 1 and 50 bp deletion in exon 1 and Insertion of the selection cassette in exon 1
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- SNAPIN
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SNAPIN also known as SNAP25-interacting protein has an approximate mass of 15 kDa and functions mechanically by interacting with the SNARE complex an important structure in vesicular trafficking. It binds directly to the SNAP25 protein facilitating synaptic vesicle docking and neurotransmitter release at the synapse. Expression of SNAPIN is widespread throughout neuronal tissues and other cell types indicating its role in cellular communication processes.
- Biological function summary
SNAPIN enhances the efficiency of synaptic transmission and vesicle fusion processes. It integrates into the SNARE complex where it stabilizes this multi-protein assembly essential for neurotransmitter release. By bridging interactions between SNAP25 and synaptic vesicles SNAPIN helps bridge essential synapse communication modulating the synaptic transmission needed for effective neuron signaling.
- Pathways
SNAPIN participates in the synaptic vesicle cycle and neurotransmitter release pathways. It works closely in tandem with proteins such as Synaptotagmin and Synaptobrevin which are vital players in the SNARE complex responsible for membrane fusion. By modulating neurotransmitter release SNAPIN contributes to synaptic plasticity and response to neurological stimuli linking it to broader neurological signaling pathways.
- Associated diseases and disorders
Dysfunction of SNAPIN can relate to neurodegenerative diseases including Alzheimer's disease due to its role in neurotransmitter release and synaptic function. Misregulation of SNAPIN function might also implicate it in psychiatric disorders such as schizophrenia where synaptic communication deficits are present. Additionally its involvement with SNAP25 in the SNARE complex associates it with these diseases and potential disruptions in SNARE-associated pathways and synaptic communication.
Product promise
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4 product images
Sanger Sequencing - Human SNAPIN knockout HeLa cell line (ab265393)
Allele-3: 35 bp deletion in exon 1.
Sanger Sequencing - Human SNAPIN knockout HeLa cell line (ab265393)
Allele-4: 29 bp deletion in exon 1.
Sanger Sequencing - Human SNAPIN knockout HeLa cell line (ab265393)
Allele-2: 50 bp deletion in exon 1.
Sanger Sequencing - Human SNAPIN knockout HeLa cell line (ab265393)
Allele-1: Insertion of the selection cassette in exon 1.
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