Human SNRPB2 knockout HeLa cell line
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SNRPB2 KO cell line available to order. KO validated. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 3. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.
View Alternative Names
2810052G09Rik, MGC24807, MGC45309, OTTHUMP00000030324, OTTHUMP00000030325, OTTMUSP00000016621, OTTMUSP00000016622, RP23-371L4.1, RU2B_HUMAN, Small nuclear ribonucleoprotein polypeptide B, Small nuclear ribonucleoprotein polypeptide B2, U2 small nuclear ribonucleoprotein B'', U2 snRNP B'', U2B''
- Sanger seq
Unknown
Sanger Sequencing - Human SNRPB2 knockout HeLa cell line (AB265100)
Homozygous : 1 bp deletion in exon 3.
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Proteins like SNRPB2 contribute to the regulation of gene expression by ensuring the accurate removal of introns from precursor mRNA. SNRPB2 exists as part of the U2 snRNP an important component of the spliceosome. This complex participates in recognizing the splicing sites and catalyzing the chemical reactions necessary for intron excision. Therefore SNRPB2 along with other proteins and RNA molecules within the spliceosome guarantees precise RNA maturation which is necessary for correct protein synthesis in eukaryotic cells.
Pathways
RNA splicing machinery like the spliceosome featuring SNRPB2 engages in the RNA processing pathway. This protein often works alongside U1 snRNP and is also interconnected with mRNA surveillance mechanisms ensuring that errors in RNA transcripts are recognized and degraded. Importantly SNRPB2 has relationships with other spliceosome components such as SF3A and SF3B that also ensure the fidelity of the splicing process and its integration with various cellular signaling pathways.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com