SNRPB2 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 3.
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Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 3
Alternative names
2810052G09Rik, MGC24807, MGC45309, OTTHUMP00000030324, OTTHUMP00000030325, OTTMUSP00000016621, OTTMUSP00000016622, RP23-371L4.1, RU2B_HUMAN, Small nuclear ribonucleoprotein polypeptide B, Small nuclear ribonucleoprotein polypeptide B2, U2 small nuclear ribonucleoprotein B'', U2 snRNP B'', U2B''
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SNRPB2 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 3.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 3
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- SNRPB2
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SNRPB2 also known as small nuclear ribonucleoprotein polypeptide B' is a protein that plays a role in the splicing of pre-mRNA. It often weighs around 25 kDa and is expressed in the nucleus of cells. The protein is a component of the spliceosome which is an essential part of the RNA processing machinery in eukaryotic cells. In particular SNRPB2 is part of the U2 small nuclear ribonucleoprotein (snRNP) complex acting in spliceosome assembly and RNA splicing events.
- Biological function summary
Proteins like SNRPB2 contribute to the regulation of gene expression by ensuring the accurate removal of introns from precursor mRNA. SNRPB2 exists as part of the U2 snRNP an important component of the spliceosome. This complex participates in recognizing the splicing sites and catalyzing the chemical reactions necessary for intron excision. Therefore SNRPB2 along with other proteins and RNA molecules within the spliceosome guarantees precise RNA maturation which is necessary for correct protein synthesis in eukaryotic cells.
- Pathways
RNA splicing machinery like the spliceosome featuring SNRPB2 engages in the RNA processing pathway. This protein often works alongside U1 snRNP and is also interconnected with mRNA surveillance mechanisms ensuring that errors in RNA transcripts are recognized and degraded. Importantly SNRPB2 has relationships with other spliceosome components such as SF3A and SF3B that also ensure the fidelity of the splicing process and its integration with various cellular signaling pathways.
- Associated diseases and disorders
Mutations or malfunctions in SNRPB2 have links with conditions like retinitis pigmentosa and spinal muscular atrophy. These genetic disorders often stem from disruptions in the normal splicing processes that are essential for nerve and photoreceptor cell function. In these disorders connections have been made between SNRPB2 and other related proteins such as SMN1 in spinal muscular atrophy further illustrating the complexity of its involvement in maintaining healthy cellular function and gene expression.
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Sanger Sequencing - Human SNRPB2 knockout HeLa cell line (ab265100)
Homozygous: 1 bp deletion in exon 3.
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