SPCS1 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 2 bp deletion in exon 1.
Images
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 2 bp deletion in exon 1
Alternative names
HSPC033, Microsomal signal peptidase 12 kDa subunit, OTTHUMP00000206959, SPC1, SPC12, SPCS1_HUMAN, SPase 12 kDa subunit, Signal peptidase complex subunit 1, YJR010C-A, signal peptidase 12kDa, signal peptidase complex subunit 1 homolog (S. cerevisiae)
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SPCS1 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 2 bp deletion in exon 1.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 2 bp deletion in exon 1
- Concentration
Properties
- Gene name
- SPCS1
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Signal peptidase complex subunit 1 (SPCS1) also known as SPC18 is a protein with an approximate mass of 22 kDa. It forms a part of the signal peptidase complex in the endoplasmic reticulum of eukaryotic cells. This protein assists in the cleavage of signal peptides from nascent proteins being synthesized. SPCS1 is ubiquitously expressed across various tissues which indicates its significance in general cellular processes.
- Biological function summary
SPCS1 supports the proper processing and maturation of secretory and membrane proteins. As a constituent of the signal peptidase complex SPCS1 participates directly in the co-translational removal of signal peptides. This functionality is integral for protein targeting and trafficking ensuring that proteins reach their intended locations within the cell or are secreted outside.
- Pathways
SPCS1 is involved in the protein processing pathway within the endoplasmic reticulum. This pathway plays a role in maintaining cellular protein homeostasis. The function of SPCS1 makes it relevant to the unfolded protein response pathway indirectly influencing pathways that involve stress response and protein folding. Proteins like SEC61 which form the translocon complex work closely with SPCS1 in these pathways to facilitate protein synthesis and translocation across the ER membrane.
- Associated diseases and disorders
Disruptions in the function of SPCS1 can relate to certain neurodegenerative diseases due to its role in protein processing. Improper protein cleavage and folding could contribute to conditions such as Alzheimer's disease. Additionally the dysregulation of SPCS1 is connected to cancer where anomalies in protein maturation pathways may lead to uncontrolled cell growth. In these contexts SPCS1 abnormalities may be associated with other proteins such as tau in Alzheimer's disease and various oncogenes or tumor suppressor proteins in cancer.
Product promise
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2 product images
Sanger Sequencing - Human SPCS1 knockout HEK-293T cell line (ab266405)
Allele-2: 1 bp insertion in exon 1.
Sanger Sequencing - Human SPCS1 knockout HEK-293T cell line (ab266405)
Allele-1: 2 bp deletion in exon 1
Downloads
Product protocols
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- Visit troubleshooting
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