Human SQLE (Squalene Epoxidase) knockout HeLa cell line
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SQLE KO cell line available to order. KO validated by. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 5 bp deletion in exon 1 and Insertion of the selection cassette in exon 1.
View Alternative Names
ERG1_HUMAN, SE, SQLE, Squalene epoxidase, Squalene monooxygenase
- Sanger seq
Unknown
Sanger Sequencing - Human SQLE (Squalene Epoxidase) knockout HeLa cell line (AB265787)
Allele-1 : 5 bp deletion in exon 1.
- Sanger seq
Unknown
Sanger Sequencing - Human SQLE (Squalene Epoxidase) knockout HeLa cell line (AB265787)
Allele-2 : Insertion of the selection cassette in exon 1.
Product details
Recommended control: Human wild-type HeLa cell line (ab255928). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Squalene epoxidase plays an important role in maintaining sterol homeostasis by regulating the conversion of squalene an important precursor in the cholesterol synthesis pathway. This enzyme operates as a part of the endoplasmic reticulum membrane complex where it anchors and acts on substrates to produce sterol intermediates for further enzymatic actions. Its function is tightly regulated by intracellular cholesterol levels to prevent overproduction and maintain cell membrane integrity.
Pathways
Squalene epoxidase is an important component of the mevalonate pathway a fundamental route for the synthesis of cholesterol and other isoprenoids. Besides cholesterol biosynthesis it connects to the synthesis of steroid hormones and bile acids. Farnesyl pyrophosphate another key intermediate in the mevalonate pathway influences the activity of squalene epoxidase. Moreover this enzyme cooperates with subsequent enzymes like lanosterol synthase to facilitate the continuation of sterol biosynthesis.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Complementary Products
Primary Antibodies
AB317629
Anti-Squalene Epoxidase antibody [EPR29195-89] - BSA and Azide free
Recombinant|RabMAb
![Western blot - Anti-Squalene Epoxidase antibody [EPR29195-89] - BSA and Azide free (AB317629)](https://content.abcam.com/products/images/squalene-epoxidase-antibody-epr29195-89-bsa-and-azide-free-ab317629--western-blot-img432802.jpg)
- 0
- 0
![Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (AB205617)](https://content.abcam.com/products/images/idi1-idi2-antibody-epr17213-ab205617--western-blot-img61370.jpg)
Cell Lines & Lysates
AB265976
Human HTT (Huntingtin) knockout HeLa cell line
Advanced Validation
- 0
- 0
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com