ZNRD2 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and Insertion of the selection cassette in exon 2.
Images
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and Insertion of the selection cassette in exon 2
Alternative names
1500016H19Rik, Autoantigen p27, C184L, OTTHUMP00000232769, OTTHUMP00000233069, OTTHUMP00000233071, SSA27_HUMAN, Sjoegren syndrome/scleroderma autoantigen 1, Sjogren syndrome/scleroderma autoantigen 1, Sjogren's syndrome/scleroderma autoantigen 1 homolog, centromeric autoantigen (27kD), p27
Recommended products
ZNRD2 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and Insertion of the selection cassette in exon 2.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and Insertion of the selection cassette in exon 2
- Concentration
Properties
- Gene name
- ZNRD2
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SSSCA1 also known as spindle and kinetochore-associated complex subunit 1 is a protein with a molecular mass of approximately 11 kDa. It plays a significant role in cell division. SSSCA1 localizes to the nucleus and associates with the mitotic spindle during cell cycle progression. Expression of SSSCA1 can vary across different tissues but it is particularly prominent in rapidly dividing cells including those in the testis and thymus.
- Biological function summary
The protein acts as a critical player in the regulation of mitosis. SSSCA1 forms part of a larger multiprotein complex essential for the proper attachment of chromosomes to the mitotic spindle. By ensuring that the chromosomes align correctly SSSCA1 facilitates accurate segregation during cell division. It works closely with other components of the complex to maintain genomic stability.
- Pathways
The protein influences critical cell division processes. SSSCA1 integrates into the spindle assembly checkpoint pathway ensuring the proper timing of mitosis. Within this pathway it often interacts with proteins such as BubR1 and Mad2 which are responsible for halting cell cycle progression until chromosomes are correctly attached to the spindle fibers. Its role in checkpoint pathways highlights its importance in maintaining orderly mitotic progression.
- Associated diseases and disorders
Defects in the expression or function of SSSCA1 have connections to cancer development. Cancer cells frequently exhibit abnormal cell division and dysregulation of SSSCA1 can exacerbate these issues. The protein's interaction with p53 a well-known tumor suppressor protein might further hint at its relevance to cancer proliferation. Additionally anomalies in the functioning of SSSCA1 have also been observed in various chromosomal instability syndromes highlighting its influence in maintaining chromosome integrity.
Product promise
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2 product images
Sanger Sequencing - Human SSSCA1 knockout HEK-293T cell line (ab266088)
Allele-1: 1 bp deletion in exon2
Sanger Sequencing - Human SSSCA1 knockout HEK-293T cell line (ab266088)
Allele-2: Insertion of the selection cassette in exon 2.
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