Human SSSCA1 knockout HEK-293T cell line
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ZNRD2 KO cell line available to order. KO validated. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and Insertion of the selection cassette in exon 2. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.
View Alternative Names
1500016H19Rik, Autoantigen p27, C184L, OTTHUMP00000232769, OTTHUMP00000233069, OTTHUMP00000233071, SSA27_HUMAN, Sjoegren syndrome/scleroderma autoantigen 1, Sjogren syndrome/scleroderma autoantigen 1, Sjogren's syndrome/scleroderma autoantigen 1 homolog, centromeric autoantigen (27kD), p27
- Sanger seq
Unknown
Sanger Sequencing - Human SSSCA1 knockout HEK-293T cell line (AB266088)
Allele-2 : Insertion of the selection cassette in exon 2.
- Sanger seq
Unknown
Sanger Sequencing - Human SSSCA1 knockout HEK-293T cell line (AB266088)
Allele-1 : 1 bp deletion in exon2
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The protein acts as a critical player in the regulation of mitosis. SSSCA1 forms part of a larger multiprotein complex essential for the proper attachment of chromosomes to the mitotic spindle. By ensuring that the chromosomes align correctly SSSCA1 facilitates accurate segregation during cell division. It works closely with other components of the complex to maintain genomic stability.
Pathways
The protein influences critical cell division processes. SSSCA1 integrates into the spindle assembly checkpoint pathway ensuring the proper timing of mitosis. Within this pathway it often interacts with proteins such as BubR1 and Mad2 which are responsible for halting cell cycle progression until chromosomes are correctly attached to the spindle fibers. Its role in checkpoint pathways highlights its importance in maintaining orderly mitotic progression.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com