Human STK4 (Serine/threonine-protein kinase 4/MST-1) knockout HeLa cell line
- Advanced Validation
- What is this?
Be the first to review this product! Submit a review
|
(1 Publication)
- WB
Unknown
Western blot - Human STK4 (Serine/threonine-protein kinase 4/MST-1) knockout HeLa cell line (AB265442)
Lanes 1-4 : Merged signal (red and green). Green - ab51134 observed at 52 kDa. Red - loading control ab8245 observed at 37 kDa.
ab51134 Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] was shown to specifically react with MST-1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265442 (knockout cell lysate ab258215) was used. Wild-type and MST-1 knockout samples were subjected to SDS-PAGE. ab51134 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4° at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (<a href='/en-us/products/primary-antibodies/serine-threonine-protein-kinase-4-mst-1-antibody-ep1465y-ab51134'>ab51134</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
STK4 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human STK4 (Serine/threonine-protein kinase 4/MST-1) knockout HeLa cell line (ab265442)
Lane 3:
Jurkat cell lysate at 20 µg
Lane 4:
Ramos cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 52 kDa
false
- Sanger seq
Lab
Sanger Sequencing - Human STK4 (Serine/threonine-protein kinase 4/MST-1) knockout HeLa cell line (AB265442)
Allele-2 : Insertion of the selection cassette in exon 3.
- Sanger seq
Unknown
Sanger Sequencing - Human STK4 (Serine/threonine-protein kinase 4/MST-1) knockout HeLa cell line (AB265442)
Allele-1 : 67 bp insertion in exon 3.
Complementary Products
Primary Antibodies
AB51134
Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y]
RabMAb|Recombinant|KO Validated|20ul selling size
![Flow Cytometry (Intracellular) - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (AB51134)](https://content.abcam.com/products/images/serine-threonine-protein-kinase-4-mst-1-antibody-ep1465y-ab51134--flow-cytometry-intracellular-img27449.jpg)
- 0
- 0
Reactivity data
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This kinase orchestrates various cellular processes mainly through regulation of apoptosis and cell stress responses. MST-1 interacts with other signaling molecules in cells forming part of larger multi-protein complexes. These interactions allow MST-1 to act as a molecular switch effectively controlling the balance between cell survival and death. The cellular localization and interaction partners help diversify its biological roles.
Pathways
MST-1 predominantly influences the Hippo signaling pathway—a pathway significant in controlling organ size and suppressing cancer. In this pathway MST-1 phosphorylates and activates LATS1/2 kinases creating an endpoint effect on cell proliferation and apoptosis. MST-1 also participates in the oxidative stress response pathway where it shows interaction with FoxO transcription factors leading to modifications in gene expression profiles.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
Biomedicines 11: PubMed37893233
2023
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com