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SUV39H2 KO cell line available to order. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control provided. Knockout achieved by CRISPR/Cas9 X = 2 bp deletion Frameshift = 97%.

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Images

Western blot - Human SUV39H2 knockout U-2 OS cell line (AB261882), expandable thumbnail
  • Next Generation Sequencing - Human SUV39H2 knockout U-2 OS cell line (AB261882), expandable thumbnail

Key facts

Cell type
U-2 OS
Species or organism
Human
Tissue
Bone
Form
Liquid
Knockout validation
Next Generation Sequencing, Western blot
Mutation description
Knockout achieved by CRISPR/Cas9 X = 2 bp deletion Frameshift = 97%

Alternative names

FLJ23414, H3-K9-HMTase 2, Histone H3-K9 methyltransferase 2, Histone lysine N methyltransferase H3 lysine 9 specific 2, Histone-lysine N-methyltransferase SUV39H2, KMT1B, Lysine N-methyltransferase 1B, SUV92_HUMAN, Su(var)3 9 Drosophila homolog of 2, Su(var)3-9 homolog 2, Suppressor of variegation 3-9 homolog 2, sSuppressor of variegation 3 9 homolog 2 (Drosophila)

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SUV39H2 KO cell line available to order. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control provided. Knockout achieved by CRISPR/Cas9 X = 2 bp deletion Frameshift = 97%.

Key facts

Cell type
U-2 OS
Form
Liquid
Mutation description
Knockout achieved by CRISPR/Cas9 X = 2 bp deletion Frameshift = 97%
Disease
Osteosarcoma
Concentration
Loading...

Properties

Gene name
SUV39H2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Next Generation Sequencing, Western blot

Quality control

STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Female

Handling procedures

Initial handling guidelines

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.

Subculture guidelines
  • All seeding densities should be based on cell counts gained by established methods.
  • A guide seeding density of 2x104 cells/cm2 is recommended.
  • Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
McCoY5a + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-196°C
Appropriate long-term storage conditions
-196°C

Notes

Recommended control: Human wild-type U-2 OS cell line (ab263976). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.

We will provide viable cells that proliferate on revival.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

KMT1B also known as SUV39H2 is a lysine-specific histone methyltransferase enzyme located on chromosome 10q22.2. It catalyzes the methylation of histone H3 at lysine 9 (H3K9) which condenses chromatin into a more transcriptionally inactive state. KMT1B is approximately 50 kDa in mass and shows highest expression in testis and fetal tissues. It modifies chromatin structure to regulate gene expression patterns affecting cellular processes.

Biological function summary

This protein influences epigenetic constructs by being part of the transcriptional repressor complex. It is involved in gene silencing through the formation of heterochromatin an essential process for maintaining genome stability. KMT1B shares homology with SUV39H1 another histone methyltransferase and both cooperate in establishing heterochromatin markers.

Pathways

KMT1B participates in the transcriptional regulation pathway and intersects with pathways governing cell cycle control. It contributes to the E2F transcriptional repression pathway by interacting with retinoblastoma protein (pRB) which controls cell proliferation. This connection to pRB positions KMT1B as an influential factor in cell cycle checkpoints and progression.

Associated diseases and disorders

KMT1B mutations have associations with neurodevelopmental disorders and some cancers. Its dysregulation can lead to anomalies in chromatin compaction affecting neurological development and potentially promoting oncogenesis. In cancer changes in KMT1B expression relate to abnormal cell growth often linked with parallel alterations in related enzymes like SUV39H1.

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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