SYNJ2 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon 3 and 68 bp deletion in exon 3.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon 3 and 68 bp deletion in exon 3
Alternative names
5-trisphosphate 5-phosphatase 2, INPP 5H, Inositol phosphate 5' phosphatase 2, KIAA0348, MGC44422, SJ2, SYNJ2_HUMAN, Synaptic inositol 1 4 5 trisphosphate 5 phosphatase 2, Synaptic inositol-1, Synaptojanin-2
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SYNJ2 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon 3 and 68 bp deletion in exon 3.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon 3 and 68 bp deletion in exon 3
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- SYNJ2
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Synaptojanin 2 also known as SYNJ2 is a protein encoded by the SYNJ2 gene. It has a molecular mass of approximately 180 kDa. Synaptojanin 2 functions as a phosphoinositide phosphatase which helps dephosphorylate phosphatidylinositol 45-bisphosphate (PIP2) to phosphatidylinositol (PI). This affects the membrane dynamics and signal transduction. The expression of synaptojanin 2 occurs in various tissues with notable abundance in the brain and testis.
- Biological function summary
Synaptojanin 2 plays an important role in synaptic vesicle trafficking and endocytosis. It can function as a part of protein complexes involved in these processes. The protein is involved in rearranging the actin cytoskeleton which impacts cellular shape and movement. Its activity influences synaptic transmission efficiency by controlling the cycle of synaptic vesicles particularly in the nervous system which reflects its high expression in the brain.
- Pathways
Synaptojanin 2 is closely linked to the phosphatidylinositol signaling pathway and the endocytosis pathway. These pathways are important for cellular communication and nutrient uptake. Within these pathways synaptojanin 2 interacts with proteins like dynamin a GTPase that mediates membrane fission during endocytosis. Synaptojanin 2 also functions in relation to AP-2 a clathrin adaptor complex that plays a role in vesicle formation highlighting its participation in endocytic trafficking.
- Associated diseases and disorders
Synaptojanin 2 relates to conditions such as cancer and neurodegenerative diseases. Overexpression or mutation of synaptojanin 2 associates with certain types of cancers including breast cancer potentially via disrupted signal transduction. It maintains a connection with proteins like PTEN a known tumor suppressor through its involvement in lipid signaling. In neurodegenerative diseases abnormal synaptojanin 2 function may impact neuronal health and is linked to diseases such as Alzheimer's disease. Defective phosphoinositide metabolism influences amyloid precursor protein processing implicating synaptojanin 2 in the pathology of these disorders.
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2 product images
Sanger Sequencing - Human SYNJ2 (Synaptojanin 2) knockout HeLa cell line (ab265359)
Allele-2: 68 bp deletion in exon 3.
Sanger Sequencing - Human SYNJ2 (Synaptojanin 2) knockout HeLa cell line (ab265359)
Allele-1: 14 bp deletion in exon 3.
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