Human TAF12 knockout HeLa cell line
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TAF12 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
View Alternative Names
TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa, TAF12_HUMAN, TAF2J, TAFII-20/TAFII-15, TAFII20, Transcription initiation factor TFIID 20/15 kDa subunits, Transcription initiation factor TFIID subunit 12
- Sanger seq
Unknown
Sanger Sequencing - Human TAF12 knockout HeLa cell line (AB265818)
Homozygous : 1 bp insertion in exon 2.
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TAF12 contributes to the formation and stability of the TFIID complex which is essential for the initiation of transcription by RNA polymerase II. As part of this complex TAF12 helps recognize and bind to the core promoter elements of genes facilitating the assembly of the pre-initiation complex. This process drives gene expression that is responsive to various physiological signals. TAF12's interaction with other TAF (TBP-associated factors) proteins ensures precision in the selection of transcription start sites.
Pathways
TAF12 integrates into gene expression pathways affecting cellular proliferation and differentiation. Its role in the canonical Wnt signaling pathway is significant a pathway that regulates important aspects of development and cell signaling. TAF12 also interacts with proteins such as TAF1 and TBP which jointly influence transcription initiation. These protein interactions are fundamental for the regulation of genes that control cell cycle progression and response to external growth signals.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com