TANK KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 2.
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Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 2
Alternative names
I-TRAF, TANK_HUMAN, TRAF family member associated NFKB activator, TRAF family member-associated NF-kappa-B activator, TRAF interacting protein TANK isoform a, TRAF interacting protein TANK isoform b, TRAF-interacting protein
Recommended products
TANK KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 2.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 2
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- TANK
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TANK (TRAF family member-associated NF-kappa-B activator) and TRAF2 (TNF receptor-associated factor 2) are proteins involved in the regulation of immune responses. TANK also known as I-TRAF is a protein with a mass of approximately 49 kDa. TRAF2 has a mass of approximately 56 kDa. These proteins express in various immune cells including T and B lymphocytes. TANK interacts with TRAF2 to mediate downstream signaling pathways essential for immunity.
- Biological function summary
The TANK/TRAF2 complex plays a role in modulating the NF-kappa-B signaling pathway which is important for inflammation and immune responses. This complex also contributes to cellular responses to stress and infection. TANK acts as an adaptor protein that interacts with TRAF2 to relay signals from upstream receptors ensuring proper immune function. Additionally TANK inhibits excessive activation of NF-kappa-B maintaining a balanced immune response.
- Pathways
As it relates to regulating immune functions TANK/TRAF2 is pivotal in the NF-kappa-B and MAPK signaling pathways. In the NF-kappa-B pathway TRAF2 helps mediate the activation of downstream kinases which are critical for the translocation of NF-kappa-B to the nucleus. TANK works alongside other signaling molecules like TRAF3 and TRAF6 integrating signals that lead to gene transcription involved in immune response and cell survival.
- Associated diseases and disorders
The dysfunction of TANK/TRAF2 differs from normal regulatory controls it links to auto-inflammatory diseases and certain cancers. In autoimmune disorders such as systemic lupus erythematosus aberrant NF-kappa-B activity possibly influenced by TANK/TRAF2 leads to uncontrolled immune activation. In cancer particularly lymphomas altered TANK/TRAF2 signaling correlates with the unchecked proliferation of cells. The protein's interplay with receptor-interacting protein 1 (RIP1) further emphasizes its role as disruptions in this interaction are seen in pathological states.
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1 product image
Sanger Sequencing - Human TANK (TRAF2) knockout HeLa cell line (ab264887)
Homozygous: Insertion of the selection cassette in exon 2.
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