Human TBC1D7 knockout HeLa cell line
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TBC1D7 KO cell line available to order. KO validated by. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 19 bp deletion in exon 2 and 2 bp deletion in exon 2.
View Alternative Names
Cell Migration-Inducing Protein 23, HSPC239, PIG51, TBC1 domain family member 7, TBC7, TCD1D7, dJ257A7.3
- Sanger seq
Unknown
Sanger Sequencing - Human TBC1D7 knockout HeLa cell line (AB265559)
Allele-1 : 19 bp deletion in exon 2.
- Sanger seq
Unknown
Sanger Sequencing - Human TBC1D7 knockout HeLa cell line (AB265559)
Allele-2 : 2 bp deletion in exon 2.
Product details
Recommended control: Human wild-type HeLa cell line (ab255928). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TBC1D7 functions as a part of the TSC complex consisting of TSC1 (hamartin) TSC2 (tuberin) and TBC1D7. This complex plays a significant role in cellular growth regulation by inactivating the small GTPase Rheb. This action prevents the overactivation of mTORC1 (mechanistic target of rapamycin complex 1) an important controller of cell growth and proliferation. TBC1D7 adds stability to the TSC complex enhancing its ability to regulate mTORC1 activity effectively demonstrating its key role in cellular metabolic regulation.
Pathways
The involvement of the TBC1D7 protein in the mTOR signaling and insulin signaling pathways is well-documented. Within these pathways TBC1D7 operates along with proteins such as Rheb and mTOR itself. By affecting the TSC complex's inhibitory impact on mTORC1 TBC1D7 influences cell growth survival and metabolism. These pathways are essential for energy sensing and management impacting how cells respond to nutrient availability and growth signals.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com