TBKBP1 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 6 and 4 bp insertion in exon 6.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 6 and 4 bp insertion in exon 6
Alternative names
KIAA0775, ProSAPiP2, SINTBAD, TANK-binding kinase 1-binding protein 1, TBK1-binding protein 1, TBKB1_HUMAN
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TBKBP1 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 6 and 4 bp insertion in exon 6.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 6 and 4 bp insertion in exon 6
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- TBKBP1
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TBKBP1 also known as TANK-Binding Kinase 1 Binding Protein 1 is a protein involved in several cellular processes. It has a mass of around 61 kDa. TBKBP1 is widely expressed in various tissues including the heart brain liver and kidney. Mechanically TBKBP1 interacts with TBK1 (TANK-binding kinase 1) influencing signal transduction and the regulation of innate immune responses.
- Biological function summary
TBKBP1 functions as a modulator of immune signaling pathways. It often associates with complexes involving adaptor proteins such as TANK and IKK epsilon (IKKε). TBKBP1 plays a significant role in regulating antiviral responses by facilitating the activation of transcription factors that induce the expression of interferon-stimulated genes. These activities highlight its involvement in the regulation of the immune system’s response to viral infections.
- Pathways
TBKBP1 participates in the type I interferon signaling pathway and NF-kB signaling pathway. Within these pathways it works closely with proteins like TBK1 and IKKε contributing to cellular responses against viral and inflammatory stimuli. Through these interactions TBKBP1 helps modulate the production of cytokines and other mediators of inflammation.
- Associated diseases and disorders
TBKBP1 has connections to neurodegenerative diseases and cancer. Alterations in its normal function can result in impaired immune responses potentially contributing to the development of conditions like amyotrophic lateral sclerosis (ALS) and various forms of cancer. The interaction between TBKBP1 and TBK1 links it to pathological processes involving aberrant immune signaling and cellular stress responses.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Sanger Sequencing - Human TBKBP1 knockout HeLa cell line (ab265883)
Allele-1: 1 bp insertion in exon 6.
Sanger Sequencing - Human TBKBP1 knockout HeLa cell line (ab265883)
Allele-2: 4 bp insertion in exon 6.
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