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AB306853

Human TBX2 knockout U-87 MG cell line

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TBX2 KO cell line available to order. KO validated. Free of charge wild type control available. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.

Key facts

Cell type

U-87 MG

Species or organism

Human

Tissue

Brain

Form

Liquid

form

Disease

Glioblastoma

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Complementary Products

Primary Antibodies

AB33298

Anti-Tbx2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tbx2 antibody (AB33298)

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Product details

Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
TBX2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-196°C
Appropriate long-term storage conditions
-196°C
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Handling procedures

Initial handling guidelines
  • All seeding densities should be based on cell counts gained by established methods.
  • A guide seeding density of 2x104 cells/cm2 is recommended.
  • Cells should be passaged when they have achieved 80-90% confluence.
Subculture guidelines
  • All seeding densities should be based on cell counts gained by established methods.
  • A guide seeding density of 2x104 cells/cm2 is recommended.
  • Cells should be passaged when they have achieved 80-90% confluence.
Culture medium

EMEM + 10% FBS

Cryopreservation medium

Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tbx2 also known as T-box 2 is a transcription factor with a molecular weight of approximately 80 kDa. This protein is a member of the T-box family which is known for regulating gene expression through DNA binding. Tbx2 plays a role in controlling developmental processes by suppressing the expression of certain target genes. It is expressed in various tissues including heart limbs and craniofacial regions during embryonic development.
Biological function summary

This transcription factor is influential in cellular proliferation and developmental growth. It acts as a transcriptional repressor and participates in developmental processes such as osteogenesis and limb formation. Tbx2 is commonly found in complexes with other proteins that help modulate its effects on gene expression particularly during the early stages of embryogenesis.

Pathways

T-box 2 interacts importantly within the Wnt signaling and Sonic Hedgehog (Shh) pathways. These pathways are vital in developmental signaling where Tbx2 works with proteins such as beta-catenin in Wnt signaling influencing gene transcription linked to cell division and differentiation. Tbx2's role in these pathways affects the expression of key genes involved in tissue patterning and development.

Tbx2 has connections to cancer and developmental disorders. Its overexpression can lead to unchecked cell proliferation implicating it in melanoma and other cancers. Additionally its role in developmental processes links Tbx2 to disorders such as Holt-Oram syndrome through interactions with Tbx5 another member of the T-box family. Understanding Tbx2's precise functions and interactions is essential for developing therapeutic strategies targeting these diseases.
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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

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Product protocols

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Target data

See full target information T-box transcription factor TBX2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com