Human TCF3 knockout SW480 cell line
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TCF3 KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control available. Knockout achieved by CRISPR/Cas9 X = 10 bp deletion after Tyr107 of the WT protein Frameshift: 100%. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
View Alternative Names
AGM8, Class B basic helix-loop-helix protein 21, E12, E2A, E2A immunoglobulin enhancer binding factors E12/E47, E47, Helix loop helix protein HE47, ITF1, Immunoglobulin enhancer-binding factor E12/E47, Immunoglobulin transcription factor 1, Kappa-E2-binding factor, MGC129647, MGC129648, NOL1-TCF3 fusion, Negative vitamin D response element binding protein, TCF-3, TFE2_HUMAN, Transcription factor 3, Transcription factor E2-alpha, Transcription factor ITF-1, VDIR, VDR interacting repressor, bHLHb21, transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47), transcription factor 3 variant 3, vitamin D receptor-interacting repressor
- NGS
Lab
Next Generation Sequencing - Human TCF3 knockout SW480 cell line (AB269624)
10 bp deletion after Tyr107 of the WT protein
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2-3x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Slow growing. A partial media change is recommended at least twice between passages.
Culture medium
Ham's F-12 + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TCF3/E2A is essential for proper cellular development and differentiation. It functions as part of a larger transcription complex and partners often with other transcription factors like HEB or E47. These interactions allow E2A to regulate diverse biological processes including B cell lineage commitment and neuronal development. The ability to influence these processes highlights E2A's role in managing cellular fate and developmental outcomes.
Pathways
E2A participates actively in the Notch signaling and B-cell receptor signaling pathways. In the context of Notch signaling E2A interacts with proteins like NOTCH1 and RBPJ to influence cell lineage decisions. Meanwhile in the B-cell receptor signaling pathway E2A works closely with proteins such as PAX5 and EBF1 playing a part in B-lymphocyte proliferation and maturation. These pathways underline E2A's significant role in immune cell regulation and function.
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Target data
Complementary Products
Primary Antibodies
AB32072
Anti-c-Myc antibody [Y69] - ChIP Grade
BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc antibody [Y69] - ChIP Grade (AB32072)](https://content.abcam.com/products/images/c-myc-antibody-y69-chip-grade-ab32072--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img80839.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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