Human TCF7 knockout HeLa cell line
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TCF7 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 4 and 1 bp insertion in exon 4 and 7 bp deletion in exon 4. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
View Alternative Names
FLJ36364, MGC47735, OTTHUMP00000159391, T-cell factor 1, T-cell-specific transcription factor 1, TCF-1, TCF7 transcription factor 7, TCF7_HUMAN, Transcription factor 7, Transcription factor 7 (T cell specific HMG box), Transcription factor-7, T-cell specific, Transcription factor-7, T-cell specific, 1
- WB
Lab
Western blot - Human TCF7 knockout HeLa cell line (AB265127)
Lanes 1 - 4 : Merged signal (red and green). Green - anti-TCF7 antibody observed at 48 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.
anti-TCF7 antibody was shown to react with TCF7 in wild-type HeLa cells in Western blot with loss of signal observed in TCF7 knockout cell line ab265127 (TCF7 knockout cell lysate 258225). Wild-type HeLa and TCF7 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with anti-TCF7 antibody and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and 1/20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 h at room temperature before imaging.
All lanes:
anti-TCF7 antibody at 1/1000 dilution
Lane 1:
Wild-type Hela cell lysate at 20 µg
Lane 2:
TCF7 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human TCF7 knockout HeLa cell line (ab265127)
Lane 3:
Jurkat cell lysate at 20 µg
Lane 4:
HT29 cell lysate at 20 µg
false
- Sanger seq
Unknown
Sanger Sequencing - Human TCF7 knockout HeLa cell line (AB265127)
Allele-2 : 1 bp deletion in exon 4.
- Sanger seq
Unknown
Sanger Sequencing - Human TCF7 knockout HeLa cell line (AB265127)
Allele-3 : 1 bp insertion in exon 4.
- Sanger seq
Unknown
Sanger Sequencing - Human TCF7 knockout HeLa cell line (AB265127)
Allele-1 : 7 bp deletion in exon 4.
Reactivity data
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TCF7 regulates cell fate decisions and differentiation specifically in the T-cell lineage. It is part of a protein complex with beta-catenin and acts as a transcriptional activator. TCF7 influences the development and function of T-cells playing a critical role in adaptive immune response. It contributes to the maintenance of stem cell properties in various tissues impacting processes like cell proliferation and survival.
Pathways
TCF7 is a vital component of the Wnt/beta-catenin signaling pathway which is essential for regulating gene expression in various cellular processes. This pathway maintains homeostasis in cell proliferation differentiation and stem cell renewal. Within this signaling network beta-catenin an important mediator partners with TCF7 to modulate target gene transcription. TCF7 also interacts with other proteins such as LEF1 which similarly participates in the transcription regulation vital for cellular development.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com