Human TEAD1 knockout A549 cell line
- Advanced Validation
- What is this?
Be the first to review this product! Submit a review
|
(0 Publication)
- WB
Lab
Western blot - Human TEAD1 knockout A549 cell line (AB261901)
Lanes 1 - 2 : Merged signal (red and green). Green - ab133535 observed at 50 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab133535 was shown to react with TEF1/TEAD-1 in wild-type A549 cells in western blot with loss of signal observed in TEAD1 knockout sample. Wild-type and TEAD1 knockout A549 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab133535 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4° at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-TEF1/TEAD-1 antibody [EPR3968(2)] (<a href='/en-us/products/primary-antibodies/tef1-tead-1-antibody-epr39682-ab133535'>ab133535</a>) at 1/1000 dilution
Lane 1:
Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg
Lane 2:
TEAD1 knockout A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg
Lane 2:
Western blot - Human TEAD1 knockout A549 cell line (ab261901)
Predicted band size: 48 kDa
Observed band size: 50 kDa
false
- NGS
Supplier Data
Next Generation Sequencing - Human TEAD1 knockout A549 cell line (AB261901)
Knockout achieved by CRISPR/Cas9; X = 1 bp insertion, 2 bp insertion; Frameshift = 99.74%
Complementary Products
Primary Antibodies
AB133533
Anti-TEF1/TEAD-1 antibody [EPR3967(2)]
RabMAb|Recombinant|KO Validated|20ul selling size
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] (AB133533)](https://content.abcam.com/products/images/tef1-tead-1-antibody-epr39672-ab133533--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135181.jpg)
- 0
- 0
Primary Antibodies
AB318149
Anti-CTGF antibody [RM1199] - BSA and Azide free
Recombinant|RabMAb|KO Validated
![Flow Cytometry (Intracellular) - Anti-CTGF antibody [RM1199] - BSA and Azide free (AB318149)](https://content.abcam.com/products/images/ctgf-antibody-rm1199-bsa-and-azide-free-ab318149--flow-cytometry-intracellular-img433728.jpg)
- 0
- 0
![Western blot - Anti-PAN TEAD antibody [EPR15629] (AB197589)](https://content.abcam.com/products/images/pan-tead-antibody-epr15629-ab197589--western-blot-img424985.jpg)
Reactivity data
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Do not allow the cell density to exceed 7x104 cells/cm2.
Culture medium
F-12K + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com